ArticleJournal of bacteriology2009
Outer membrane machinery and alginate synthesis regulators control membrane vesicle production in Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 84 citations in OpenAlex.
- Helicobacter pylori-derived outer membrane vesicles: Pathogenic roles, microbiota interactions, and biomedical applications.Journal of advanced research · 2026Review
- Frontiers in bacterial outer membrane vesicles: From basic characteristics to cancer therapy.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2026Article
- Specific role of two NlpC/P60 endopeptidases in cell division and membrane vesicle formation inCurrent research in microbial sciences · 2026Article
- Antigen 43 associated withMicrobiology spectrum · 2025Article
- Engineering Versatile Bacteria-Derived Outer Membrane Vesicles: An Adaptable Platform for Advancing Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Prokaryotic microvesicles Ortholog of eukaryotic extracellular vesicles in biomedical fields.Cell communication and signaling : CCS · 2024Review
- Bacterial Membrane Vesicles: Physiological Roles, Infection Immunology, and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.Microbiology spectrum · 2023Article
- Outer Membrane Vesicles (OMVs) as Biomedical Tools and Their Relevance as Immune-Modulating Agents againstInternational journal of molecular sciences · 2023Review
- Proteolytic Activity of DegP Is Required for theMicrobiology spectrum · 2023Article
- Identification of genes involved in enhanced membrane vesicle formation inFrontiers in microbiology · 2023Article
- Plasmid-mediated virulence inFrontiers in cellular and infection microbiology · 2023Review
- Outer Membrane Vesicles: Biogenesis, Functions, and Issues.Microbiology and molecular biology reviews : MMBR · 2022Review
- The extracellular vesicle generation paradox: a bacterial point of view.The EMBO journal · 2021Review
- Bacterial Extracellular Vesicles in Gastrointestinal Tract Cancer: An Unexplored Territory.Cancers · 2021Review
- Incorporation ofFrontiers in microbiology · 2021Article
- Outer Membrane Vesicles (OMVs) Produced by Gram-Negative Bacteria: Structure, Functions, Biogenesis, and Vaccine Application.BioMed research international · 2021Review
- Multilamellar and Multivesicular Outer Membrane Vesicles Produced by a Buttiauxella agrestisApplied and environmental microbiology · 2020Article
- Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa.mBio · 2020Article
- Membrane Vesicle Production as a Bacterial Defense Against Stress.Frontiers in microbiology · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer membrane protein (OMP) assembly, resulted in hypervesiculation. In this study, we showed that the outer membrane machinery and alginate synthesis regulatory machinery are closely related to MV production in P. aeruginosa. Depletion of Opr86 resulted in increased expression of the periplasmic serine protease MucD, suggesting that the accumulation of misfolded OMPs in the periplasm is related to MV production. Indeed, the mucD mutant showed a mucoid phenotype and the mucD mutation caused increased MV production. Strains with the gene encoding alginate synthetic regulator AlgU, MucA, or MucB deleted also caused altered MV production. Overexpression of either MucD or AlgW serine proteases resulted in decreased MV production, suggesting that proteases localized in the periplasm repress MV production in P. aeruginosa. Deletion of mucD resulted in increased MV proteins, even in strains with mutations in the Pseudomonas quinolone signal (PQS), which serves as a positive regulator of MV production. This study suggests that misfolded OMPs may be important for MV production, in addition to PQS, and that these regulators act in independent pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.