ArticleThe Journal of urology2009
Multiplex pathogen identification for polymicrobial urinary tract infections using biosensor technology: a prospective clinical study.
Article in The Journal of urology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00591240 (A Biochip for Rapid Diagnosis of Complicated Urinary Tract Infection), which is not on this map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Biochip for Rapid Diagnosis of Complicated Urinary Tract Infection
Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Urinary catheter-associated microbiota change in accordance with treatment and infection status.PloS one · 2017Trial
- A biosensor platform for rapid antimicrobial susceptibility testing directly from clinical samples.The Journal of urology · 2011Trial
- Assessment of UTI Diagnostic Techniques Using the Fuzzy-PROMETHEE Model.Diagnostics (Basel, Switzerland) · 2023Article
- Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics.Accounts of chemical research · 2022Article
- Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing.Molecules (Basel, Switzerland) · 2020Review
- Non-Invasive Electrochemical Biosensors Operating in Human Physiological Fluids.Sensors (Basel, Switzerland) · 2020Review
- Nanotube assisted microwave electroporation for single cell pathogen identification and antimicrobial susceptibility testing.Nanomedicine : nanotechnology, biology, and medicine · 2019Article
- New and developing diagnostic technologies for urinary tract infections.Nature reviews. Urology · 2017Review
- Integrated Biosensor Assay for Rapid Uropathogen Identification and Phenotypic Antimicrobial Susceptibility Testing.European urology focus · 2017Article
- Electrokinetic stringency control in self-assembled monolayer-based biosensors for multiplex urinary tract infection diagnosis.Nanomedicine : nanotechnology, biology, and medicine · 2014Article
- Better tests, better care: improved diagnostics for infectious diseases.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2013Review
- An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification.The Analyst · 2013Article
- Recent advances in molecular medicine techniques for the diagnosis, prevention, and control of infectious diseases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Review
- Detection and validation of volatile metabolic patterns over different strains of two human pathogenic bacteria during their growth in a complex medium using multi-capillary column-ion mobility spectrometry (MCC-IMS).Applied microbiology and biotechnology · 2013Article
- Rapid antimicrobial susceptibility testing by sensitive detection of precursor rRNA using a novel electrochemical biosensing platform.Antimicrobial agents and chemotherapy · 2013Article
- In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing.Analytical chemistry · 2012Article
- Biosensor diagnosis of urinary tract infections: a path to better treatment?Trends in pharmacological sciences · 2011Article
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- Electrothermal Fluid Manipulation of High-Conductivity Samples for Laboratory Automation Applications.JALA (Charlottesville, Va.) · 2010Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeRapid diagnosis of urinary tract infection would have a significant beneficial impact on clinical management, particularly in patients with structural or functional urinary tract abnormalities who are highly susceptible to recurrent polymicrobial infections. We examined the analytical validity of an electrochemical biosensor array for rapid molecular diagnosis of urinary tract infection in a prospective clinical study in patients with neurogenic bladder. MATERIALS AND
methodsThe electrochemical biosensor array was functionalized with DNA probes against 16S rRNA of the most common uropathogens. Spinal cord injured patients at a Veterans Affairs hospital were recruited into the study. Urine samples were generally tested on the biosensor within 1 to 2 hours of collection. Biosensor results were compared with those obtained using standard clinical microbiology laboratory methods.
resultsWe successfully developed a 1-hour biosensor assay for multiplex identification of pathogens. From July 2007 to December 2008 we recruited 116 patients, yielding a total of 109 urine samples suitable for analysis and comparison between biosensor assay and standard urine culture. Of the samples 74% were positive, of which 42% were polymicrobial. We identified 20 organisms, of which Escherichia coli, Pseudomonas aeruginosa and Enterococcus species were the most common. Biosensor assay specificity and positive predictive value were 100%. Pathogen detection sensitivity was 89%, yielding a 76% negative predictive value.
conclusionsTo our knowledge we report the first prospective clinical study to successfully identify pathogens within a point of care time frame using an electrochemical biosensor platform. Additional efforts to improve the limit of detection and probe design are needed to further enhance assay sensitivity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.