Evidence map›Paper›PMID 19836954›Full record

ReviewTrends in cell biology2009

Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging.

Jennifer Lippincott-Schwartz, George H Patterson

Abstract readReview
In one paragraph

Review in Trends in cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.

0numbers the graph read from it
0cells of the map it votes in
144citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

144 citing papers in PubMed.

  1. Article
  2. Review
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  6. Article
  7. Review
  8. Article
  9. Article
  10. Review
  11. Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023
    Review
  12. Article
  13. Cyanine Phototruncation Enables Spatiotemporal Cell Labeling.Journal of the American Chemical Society · 2022
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article

84 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Jennifer Lippincott-SchwartzCell Biology and Metabolism Program, NICHD, NIH Bethesda, MD, USA. jlippin@helix.nih.gov
George H Patterson

Funding

Organization and Dynamics of Endomembrane Pathways and OrganellesZIAHD001609 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI BONIFACINO, JUAN · 2009 to 2019
$16.2M
BiophotonicsZIAEB000071 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI PATTERSON, GEORGE · 2010 to 2022
$9.7M
Development of green fluorescent protein technologyZIAHD008850 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI LIPPINCOTT-SCHWARTZ, JENNIFER · 2009 to 2016
$6.5M
Development of green fluorescent protein technologyZ01HD008850 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI LIPPINCOTT-SCHWARTZ, JENNIFER · 2007 to 2008
$854k
Intramural NIH HHS Z01 HD008850
6 · The paper itself

Abstract

Photoactivatable fluorescent proteins (PA-FPs) are molecules that switch to a new fluorescent state in response to activation to generate a high level of contrast. Over the past eight years, several types of PA-FPs have been developed. The PA-FPs fluoresce green or red, or convert from green to red in response to activating light. Others reversibly switch between 'off' and 'on' in response to light. The optical "highlighting" capability of PA-FPs has led to the rise of novel imaging techniques providing important new biological insights. These range from in cellulo pulse-chase labeling for tracking subpopulations of cells, organelles or proteins under physiological settings, to super-resolution imaging of single molecules for determining intracellular protein distributions at nanometer precision. This review surveys the expanding array of PA-FPs, including their advantages and disadvantages, and highlights their use in novel imaging methodologies.

Indexed as

Cytological TechniquesPhotochemical ProcessesAnimalsLuminescent ProteinsMolecular ImagingLuminescent Proteins

Identifiers

PMID19836954
PMCPMC3663713

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.