ArticleBiochimica et biophysica acta2010
Quantitative mass spectrometry of diabetic kidney tubules identifies GRAP as a novel regulator of TGF-beta signaling.
Article in Biochimica et biophysica acta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
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- MNK2 governs the macrophage antiinflammatory phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Autoantibodies targeting glomerular annexin A2 identify patients with proliferative lupus nephritis.Proteomics. Clinical applications · 2015Article
- Human MCS5A1 candidate breast cancer susceptibility gene FBXO10 is induced by cellular stress and correlated with lens epithelium-derived growth factor (LEDGF).Molecular carcinogenesis · 2014Article
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- Proteomics and systems biology for understanding diabetic nephropathy.Journal of cardiovascular translational research · 2012Review
- An astrocyte-specific proteomic approach to inflammatory responses in experimental rat glaucoma.Investigative ophthalmology & visual science · 2012Article
- Elongin C is a mediator of Notch4 activity in human renal tubule cells.Biochimica et biophysica acta · 2011Article
- Neurodegenerative and inflammatory pathway components linked to TNF-α/TNFR1 signaling in the glaucomatous human retina.Investigative ophthalmology & visual science · 2011Article
- Transcriptome analysis of human diabetic kidney disease.Diabetes · 2011Article
- Identification of phosphoproteins associated with human neutrophil granules following chemotactic peptide stimulation.Molecular & cellular proteomics : MCP · 2011Article
- RASSF1A and the rs2073498 Cancer Associated SNP.Frontiers in oncology · 2011Article
- Glaucomatous tissue stress and the regulation of immune response through glial Toll-like receptor signaling.Investigative ophthalmology & visual science · 2010Article
- Oxidative stress and the regulation of complement activation in human glaucoma.Investigative ophthalmology & visual science · 2010Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The aim of this study was to define novel mediators of tubule injury in diabetic kidney disease. For this, we used state-of-the-art proteomic methods combined with a label-free quantitative strategy to define protein expression differences in kidney tubules from transgenic OVE26 type 1 diabetic and control mice. The analysis was performed with diabetic samples that displayed a pro-fibrotic phenotype. We have identified 476 differentially expressed proteins. Bioinformatic analysis indicated several clusters of regulated proteins in relevant functional groups such as TGF-beta signaling, tight junction maintenance, oxidative stress, and glucose metabolism. Mass spectrometry detected expression changes of four physiologically relevant proteins were confirmed by immunoblot analysis. Of these, the Grb2-related adaptor protein (GRAP) was up-regulated in kidney tubules from diabetic mice and fibrotic kidneys from diabetic patients, and subsequently confirmed as a novel component of TGF-beta signaling in cultured human renal tubule cells. Thus, indicating a potential novel role for GRAP in TGF-beta-induced tubule injury in diabetic kidney disease. Although we targeted a specific disease, this approach offers a robust, high-sensitivity methodology that can be applied to the discovery of novel mediators for any experimental or disease condition.
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