ArticleProtein expression and purification2010
Expression plasmids and production of EGFP in stably transfected Acanthamoeba.
Article in Protein expression and purification, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Genome Sequence Data Reveal Complex and Variable Ploidy in the Amoebozoan Acanthamoeba castellanii.Genome biology and evolution · 2026Article
- High-efficiency transfection of Acanthamoeba castellanii using a cationic polymer.Cell reports methods · 2026Article
- The fate of artificial transgenes in Acanthamoeba castellanii.BMC genomics · 2025Article
- Targets for the diagnosis ofmSphere · 2025Article
- Establishing a Cre/loxP-based genetic manipulation system for Acanthamoeba: Targeted genome editing and stable reporter expression.Parasites, hosts and diseases · 2025Article
- Article
- Recombinant protein expression inFrontiers in bioengineering and biotechnology · 2025Article
- Cellulose binding and the timing of expression influence protein targeting to the double-layered cyst wall ofmSphere · 2024Article
- Phylogenomic, structural, and cell biological analyses reveal thatMicrobiology spectrum · 2024Article
- Genetic manipulation of giant viruses and their host, Acanthamoeba castellanii.Nature protocols · 2024Review
- Glucose metabolism in the pathogenic free-living amoebae: Tempting targets for treatment development.Chemical biology & drug design · 2024Review
- Evolution of giant pandoravirus revealed by CRISPR/Cas9.Nature communications · 2023Article
- Role of an FNIP Repeat Domain-Containing Protein Encoded by Megavirus Baoshan during Viral Infection.Journal of virology · 2022Article
- Article
- An unusual thioredoxin system in the facultative parasite Acanthamoeba castellanii.Cellular and molecular life sciences : CMLS · 2021Article
- Cytochrome P450 monooxygenase of Acanthamoeba castellanii participates in resistance to polyhexamethylene biguanide treatment.Parasite (Paris, France) · 2021Article
- The role of the Acanthamoeba castellanii Sir2-like protein in the growth and encystation of Acanthamoeba.Parasites & vectors · 2020Article
- Encystment Induces Down-Regulation of an Acetyltransferase-Like Gene inPathogens (Basel, Switzerland) · 2020Article
- The most abundant cyst wall proteins of Acanthamoeba castellanii are lectins that bind cellulose and localize to distinct structures in developing and mature cyst walls.PLoS neglected tropical diseases · 2019Article
- Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
New plasmids containing the TATA-Binding Protein (TBP), TBP Promoter Binding Factor (TPBF) or Glyceraldehyde Phosphate Dehydrogenase (GAPDH) gene promoters from Acanthamoeba castellanii are described. The promoters for Acanthamoeba TPBF and GAPDH genes were used to drive constitutive expression of enhanced green fluorescent protein (EGFP) in stably transfected Acanthamoeba. Based initially on fluorescence microscopy and SDS-PAGE analysis of EGFP, both promoters produce robust expression of EGFP, with the highest level obtained from the GAPDH gene promoter in cells grown in low concentrations of neomycin G418. Purification of EGFP from lysates of 22-ml cultures by conventional chromatography yielded approximately 1.1mg of EGFP, a value that extrapolates to 50mg per liter of cell culture. The results suggest that Acanthamoeba is a useful cost-effective system for the production of recombinant proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.