ArticleBMC molecular biology2009
A small intergenic region drives exclusive tissue-specific expression of the adjacent genes in Arabidopsis thaliana.
Article in BMC molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 26 citations in OpenAlex.
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- Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.BMC plant biology · 2024Article
- Dissecting the Role of Promoters of Pathogen-sensitive Genes in Plant Defense.Current genomics · 2020Review
- Analysis of Promoters of Arabidopsis thaliana Divergent Gene Pair SERAT3;2 and IDH-III Shows SERAT3;2 Promoter is Nested Within the IDH-III Promoter.Molecular biotechnology · 2017Article
- Deep analysis of wild Vitis flower transcriptome reveals unexplored genome regions associated with sex specification.Plant molecular biology · 2017Article
- Histone modifications facilitate the coexpression of bidirectional promoters in rice.BMC genomics · 2016Article
- Functional characterization of open chromatin in bidirectional promoters of rice.Scientific reports · 2016Article
- A low-temperature-responsive element involved in the regulation of the Arabidopsis thaliana At1g71850/At1g71860 divergent gene pair.Plant cell reports · 2016Article
- The intergenic region of the maize defensin-like protein genes Def1 and Def2 functions as an embryo-specific asymmetric bidirectional promoter.Journal of experimental botany · 2016Article
- Rapid method for DNA isolation from a tough cell wall green alga Tetraspora sp. CU2551.World journal of microbiology & biotechnology · 2016Article
- Isolation and Functional Characterization of Bidirectional Promoters in Rice.Frontiers in plant science · 2016Article
- Genome-wide characterization of intergenic polyadenylation sites redefines gene spaces in Arabidopsis thaliana.BMC genomics · 2015Article
- Identification and functional characterization of bidirectional gene pairs and their intergenic regions in maize.BMC genomics · 2014Article
- Bidirectional promoters in seed development and related hormone/stress responses.BMC plant biology · 2013Article
- Article
- Genomic organisation of the Mal d 1 gene cluster on linkage group 16 in apple.Molecular breeding : new strategies in plant improvement · 2012Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundTranscription initiation by RNA polymerase II is unidirectional from most genes. In plants, divergent genes, defined as non-overlapping genes organized head-to-head, are highly represented in the Arabidopsis genome. Nevertheless, there is scarce evidence on functional analyses of these intergenic regions. The At5g06290 and At5g06280 loci are head-to-head oriented and encode a chloroplast-located 2-Cys peroxiredoxin B (2CPB) and a protein of unknown function (PUF), respectively. The 2-Cys peroxiredoxins are proteins involved in redox processes, they are part of the plant antioxidant defence and also act as chaperons. In this study, the transcriptional activity of a small intergenic region (351 bp) shared by At5g06290 and At5g06280 in Arabidopsis thaliana was characterized.
resultsActivity of the intergenic region in both orientations was analyzed by driving the beta-glucuronidase (GUS) reporter gene during the development and growth of Arabidopsis plants under physiological and stressful conditions. Results have shown that this region drives expression either of 2cpb or puf in photosynthetic or vascular tissues, respectively. GUS expression driven by the promoter in 2cpb orientation was enhanced by heat stress. On the other hand, the promoter in both orientations has shown similar down-regulation of GUS expression under low temperatures and other stress conditions such as mannitol, oxidative stress, or fungal elicitor.
conclusionThe results from this study account for the first evidence of an intergenic region that, in opposite orientation, directs GUS expression in different spatially-localized Arabidopsis tissues in a mutually exclusive manner. Additionally, this is the first demonstration of a small intergenic region that drives expression of a gene whose product is involved in the chloroplast antioxidant defence such as 2cpb. Furthermore, these results contribute to show that 2cpb is related to the heat stress defensive system in leaves and roots of Arabidopsis thaliana.
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