ArticleAnalytical chemistry2009
MALDI mass spectrometric imaging using the stretched sample method to reveal neuropeptide distributions in aplysia nervous tissue.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Sequential MALDI and Nano-DESI MS Imaging of Lipids and Proteins on Stretched Tissue Samples.Analytical chemistry · 2026Article
- Protocol for reconstructing 2D and 3D peptide atlases of the Ciona cerebral ganglion at single-cell resolution.STAR protocols · 2026Article
- Two- and three-dimensional neuropeptidomic landscape in the central nervous system of an invertebrate chordate,iScience · 2025Article
- Recent advances in mass spectrometry analysis of neuropeptides.Mass spectrometry reviews · 2023Review
- Neuropeptidomics: Improvements in Mass Spectrometry Imaging Analysis and Recent Advancements.Current protein & peptide science · 2021Review
- Genetically Encoded Fluorescent Proteins Enable High-Throughput Assignment of Cell Cohorts Directly from MALDI-MS Images.Analytical chemistry · 2019Article
- Precast Gelatin-Based Molds for Tissue Embedding Compatible with Mass Spectrometry Imaging.Analytical chemistry · 2017Article
- Classification of Large Cellular Populations and Discovery of Rare Cells Using Single Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.Analytical chemistry · 2015Article
- Metabolite localization by atmospheric pressure high-resolution scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging in whole-body sections and individual organs of the rove beetle Paederus riparius.Analytical and bioanalytical chemistry · 2015Article
- Challenges and recent advances in mass spectrometric imaging of neurotransmitters.Bioanalysis · 2014Review
- Small-volume analysis of cell-cell signaling molecules in the brain.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2014Review
- Function-driven discovery of neuropeptides with mass spectrometry-based tools.Protein and peptide letters · 2013Review
- Comparison of NIMS and MALDI platforms for neuropeptide and lipid mass spectrometric imaging inAnalytical methods : advancing methods and applications · 2013Article
- Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry.Journal of proteomics · 2012Review
- Mass spectrometric elucidation of the neuropeptidome of a crustacean neuroendocrine organ.Peptides · 2012Article
- Stretched tissue mounting for MALDI mass spectrometry imaging.Analytical chemistry · 2011Article
- L-DOPA-induced dyskinesia is associated with regional increase of striatal dynorphin peptides as elucidated by imaging mass spectrometry.Molecular & cellular proteomics : MCP · 2011Article
- MALDI mass spectrometry imaging of neuronal cell cultures.Journal of the American Society for Mass Spectrometry · 2011Article
- Revisiting rat spermatogenesis with MALDI imaging at 20-microm resolution.Molecular & cellular proteomics : MCP · 2011Article
- Neuropeptidomics: mass spectrometry-based qualitative and quantitative analysis.Methods in molecular biology (Clifton, N.J.) · 2011Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Neuropeptides are a diverse set of complex cell-cell signaling molecules that modulate behavior, learning, and memory. Their spatially heterogeneous distributions, large number of post-translational modifications, and wide range of physiologically active concentrations make their characterization challenging. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometric imaging is well-suited to characterizing and mapping neuropeptides in the central nervous system. Because matrix application can cause peptide migration within tissue samples, application parameters for MALDI typically represent a compromise between attaining the highest signal quality and preserving native spatial distributions. The stretched sample approach minimizes this trade-off by fragmenting the tissue section into thousands of spatially isolated islands, each approximately 40 mum in size. This inhibits analyte migration between the pieces and, at the same time, reduces analyte-salt adduct formation. Here, we present methodological improvements that enable the imaging of stretched tissues and reveal neuropeptide distributions in nervous tissue from Aplysia californica. The distributions of known neuropeptides are shown to correspond with previous immunohistochemical results, demonstrating that the stretched imaging method is well-suited for working with easily redistributed molecules and heterogeneous tissues and reduces adducts from physiological salts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.