ArticleProteomics2009
Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins.
Article in Proteomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed.
- Structural insights into the disulfide isomerase and chaperone activity of TrbB of the F plasmid type IV secretion system.Current research in structural biology · 2024Article
- Global mapping of Salmonella enterica-host protein-protein interactions during infection.Cell host & microbe · 2021Article
- Towards a unified open access dataset of molecular interactions.Nature communications · 2020Review
- Spatiotemporal Restriction ofThe Plant cell · 2020Article
- A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions.Journal of visualized experiments : JoVE · 2018Article
- Making the Right Choice: Critical Parameters of the Y2H Systems.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci.Frontiers in plant science · 2017Article
- Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions.PloS one · 2017Article
- TheBiochemistry and biophysics reports · 2016Article
- Bacterial protein networks: properties and functions.Nature reviews. Microbiology · 2015Review
- A yeast two-hybrid approach for probing protein-protein interactions at the centrosome.Methods in cell biology · 2015Article
- Mapping the Protein-Protein Interactome Networks Using Yeast Two-Hybrid Screens.Advances in experimental medicine and biology · 2015Review
- Article
- A second-generation protein-protein interaction network of Helicobacter pylori.Molecular & cellular proteomics : MCP · 2014Article
- The protein interaction network of bacteriophage lambda with its host, Escherichia coli.Journal of virology · 2013Article
- Systems biology approaches for discovering biomarkers for traumatic brain injury.Journal of neurotrauma · 2013Review
- Development and application of a DNA microarray-based yeast two-hybrid system.Nucleic acids research · 2013Article
- The evolutionary dynamics of protein-protein interaction networks inferred from the reconstruction of ancient networks.PloS one · 2013Article
- Popular computational methods to assess multiprotein complexes derived from label-free affinity purification and mass spectrometry (AP-MS) experiments.Molecular & cellular proteomics : MCP · 2013Review
- A comparison and optimization of yeast two-hybrid systems.Methods (San Diego, Calif.) · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Yeast two-hybrid screens often produce vastly non-overlapping interaction data when the screens are conducted in different laboratories, or use different vectors, strains, or reporter genes. Here we investigate the underlying reasons for such inconsistencies and compare the effect of seven different vectors and their yeast two-hybrid interactions. Genome-wide array screens with 49 motility-related baits from Treponema pallidum yielded 77 and 165 interactions with bait vectors pLP-GBKT7 and pAS1-LP, respectively, including 21 overlapping interactions. In addition, 90 motility-related proteins from Escherichia coli were tested in all pairwise combinations and yielded 140 interactions when tested with pGBKT7g/pGADT7g vectors but only 47 when tested with pDEST32/pDEST22. We discuss the factors that determine these effects, including copy number, the nature of the fusion protein, and species-specific differences that explain non-conserved interactions among species. The pDEST22/pDEST32 vectors produce a higher fraction of interactions that are conserved and that are biologically relevant when compared with the pGBKT7/pGADT7-related vectors, but the latter appear to be more sensitive and thus detect more interactions overall.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.