ArticlePLoS genetics2009
A Pol V-mediated silencing, independent of RNA-directed DNA methylation, applies to 5S rDNA.
Article in PLoS genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 23 citations in OpenAlex.
- Presence and function of small RNAs during plant reproduction.RNA biology · 2026Review
- From RdDM to plant defence.Nature plants · 2024Article
- An RdDM-independent function of Pol V transcripts in gene regulation and plant defence.Nature plants · 2024Article
- Thousands of high-quality sequencing samples fail to show meaningful correlation between 5S and 45S ribosomal DNA arrays in humans.Scientific reports · 2021Article
- Genetic and epigenetic variation in 5S ribosomal RNA genes reveals genome dynamics in Arabidopsis thaliana.Nucleic acids research · 2018Article
- Transcriptional Gene Silencing Maintained by OTS1 SUMO Protease Requires a DNA-Dependent Polymerase V-Dependent Pathway.Plant physiology · 2017Article
- Molecular mechanisms governing differential robustness of development and environmental responses in plants.Annals of botany · 2016Review
- The role of the Arabidopsis Exosome in siRNA-independent silencing of heterochromatic loci.PLoS genetics · 2013Article
- Pinpointing a puzzling polymerase.Nature structural & molecular biology · 2012Article
- Seeing the forest for the trees: a wide perspective on RNA-directed DNA methylation.Genes & development · 2012Article
- The RNA silencing enzyme RNA polymerase v is required for plant immunity.PLoS genetics · 2011Article
- Multisubunit RNA polymerases IV and V: purveyors of non-coding RNA for plant gene silencing.Nature reviews. Molecular cell biology · 2011Review
- RNA-mediated trans-communication can establish paramutation at the b1 locus in maize.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant-specific RNA polymerases Pol IV and Pol V are essential to RNA-directed DNA methylation (RdDM), which also requires activities from RDR2 (RNA-Dependent RNA Polymerase 2), DCL3 (Dicer-Like 3), AGO4 (Argonaute), and DRM2 (Domains Rearranged Methyltransferase 2). RdDM is dedicated to the methylation of target sequences which include transposable elements, regulatory regions of several protein-coding genes, and 5S rRNA-encoding DNA (rDNA) arrays. In this paper, we have studied the expression of the 5S-210 transcript, a marker of silencing release at 5S RNA genes, to show a differential impact of RNA polymerases IV and V on 5S rDNA arrays during early development of the plant. Using a combination of molecular and cytological assays, we show that Pol IV, RDR2, DRM2, and Pol V, actors of the RdDM, are required to maintain a transcriptional silencing of 5S RNA genes at chromosomes 4 and 5. Moreover, we have shown a derepression associated to chromatin decondensation specific to the 5S array from chromosome 4 and restricted to the Pol V-loss of function. In conclusion, our results highlight a new role for Pol V on 5S rDNA, which is RdDM-independent and comes specifically at chromosome 4, in addition to the RdDM pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.