ArticleProceedings of the National Academy of Sciences of the United States of America2009
Methionine oxidation contributes to bacterial killing by the myeloperoxidase system of neutrophils.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 153 citations in OpenAlex.
- Selenium and Selenoproteins in Neutrophil Functions.Biological trace element research · 2026Review
- Studies of the membrane-bound flavocytochrome MsrQ flavin mononucleotide (FMN)-binding site reveal an unexpected ubiquinone cofactor.The FEBS journal · 2025Article
- Beyond anaerobic respiration-new physiological roles for DmsABC and other S-/N-oxide reductases inJournal of bacteriology · 2025Article
- Antimicrobial Tolerance inAnimals : an open access journal from MDPI · 2024Review
- Stress Conditions Affect the Immunomodulatory Potential ofInternational journal of molecular sciences · 2023Article
- Neutrophil defect and lung pathogen selection in cystic fibrosis.Journal of leukocyte biology · 2023Article
- Disruption of Pseudomonas aeruginosa Adherent Cells by NaCl and NaOCl in Drinking Water.Current microbiology · 2023Article
- The formation and function of the neutrophil phagosome.Immunological reviews · 2023Review
- Periplasmic methionine sulfoxide reductase (MsrP)-a secondary factor in stress survival and virulence of Salmonella Typhimurium.FEMS microbiology letters · 2023Article
- Mechanisms of chlorate toxicity and resistance in Pseudomonas aeruginosa.Molecular microbiology · 2022Article
- Oxidation of bacillithiol during killing of Staphylococcus aureus USA300 inside neutrophil phagosomes.Journal of leukocyte biology · 2022Article
- Scylla, Charybdis, and navigating antimicrobial action in the neutrophil phagosome.Journal of leukocyte biology · 2022Article
- Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains.Journal of proteome research · 2022Article
- Article
- Discrimination of Methionine Sulfoxide and Sulfone by Human Neutrophil Elastase.Molecules (Basel, Switzerland) · 2021Article
- Tracking oxidation-induced alterations in fibrin clot formation by NMR-based methods.Scientific reports · 2021Article
- Staphylococcus aureus Peptide Methionine Sulfoxide Reductases Protect from Human Whole-Blood Killing.Infection and immunity · 2021Article
- An Extracytoplasmic Function Sigma/Anti-Sigma Factor System Regulates Hypochlorous Acid Resistance and Impacts Expression of the Type IV Secretion System inJournal of bacteriology · 2021Article
- High-altitude hypoxia induced reactive oxygen species generation, signaling, and mitigation approaches.International journal of biometeorology · 2021Review
- Deletion of both methionine sulfoxide reductase A and methionine sulfoxide reductase C genes renders Salmonella Typhimurium highly susceptible to hypochlorite stress and poultry macrophages.Molecular biology reports · 2021Article
20 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Reactive oxygen intermediates generated by neutrophils kill bacteria and are implicated in inflammatory tissue injury, but precise molecular targets are undefined. We demonstrate that neutrophils use myeloperoxidase (MPO) to convert methionine residues of ingested Escherichia coli to methionine sulfoxide in high yield. Neutrophils deficient in individual components of the MPO system (MPO, H(2)O(2), chloride) exhibited impaired bactericidal activity and impaired capacity to oxidize methionine. HOCl, the principal physiologic product of the MPO system, is a highly efficient oxidant for methionine, and its microbicidal effects were found to correspond linearly with oxidation of methionine residues in bacterial cytosolic and inner membrane proteins. In contrast, outer envelope proteins were initially oxidized without associated microbicidal effect. Disruption of bacterial methionine sulfoxide repair systems rendered E. coli more susceptible to killing by HOCl, whereas over-expression of a repair enzyme, methionine sulfoxide reductase A, rendered them resistant, suggesting a direct role for methionine oxidation in bactericidal activity. Prominent among oxidized bacterial proteins were those engaged in synthesis and translocation of peptides to the cell envelope, an essential physiological function. Moreover, HOCl impaired protein translocation early in the course of bacterial killing. Together, our findings indicate that MPO-mediated methionine oxidation contributes to bacterial killing by neutrophils. The findings further suggest that protein translocation to the cell envelope is one important pathway targeted for damage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.