ArticleThe Journal of biological chemistry2009
Functional characterization of intracellular Dictyostelium discoideum P2X receptors.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Ion Signaling in Cell Motility and Development inBiomolecules · 2024Review
- Epithelial wound healing in Clytia hemisphaerica provides insights into extracellular ATP signaling mechanisms and P2XR evolution.Scientific reports · 2023Article
- The old and the new about the contractile vacuole of Trypanosoma cruzi.The Journal of eukaryotic microbiology · 2022Review
- A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity ineLife · 2021Article
- A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.Biology open · 2017Article
- Adaptations to High Salt in a Halophilic Protist: Differential Expression and Gene Acquisitions through Duplications and Gene Transfers.Frontiers in microbiology · 2017Article
- Intracellular expression of purinoceptors.Purinergic signalling · 2015Article
- Evidence for Extracellular ATP as a Stress Signal in a Single-Celled Organism.Eukaryotic cell · 2015Article
- Rab11 regulates trafficking of trans-sialidase to the plasma membrane through the contractile vacuole complex of Trypanosoma cruzi.PLoS pathogens · 2014Article
- Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel.Nature cell biology · 2014Article
- Primitive ATP-activated P2X receptors: discovery, function and pharmacology.Frontiers in cellular neuroscience · 2013Review
- The trafficking and targeting of P2X receptors.Frontiers in cellular neuroscience · 2013Review
- Functional properties of five Dictyostelium discoideum P2X receptors.The Journal of biological chemistry · 2013Article
- The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.Cold Spring Harbor protocols · 2013Article
- Intracellular P2X receptors as novel calcium release channels and modulators of osmoregulation in Dictyostelium: a comparison of two common laboratory strains.Channels (Austin, Tex.) · 2013Article
- New insights into roles of acidocalcisomes and contractile vacuole complex in osmoregulation in protists.International review of cell and molecular biology · 2013Review
- Molecular and functional properties of P2X receptors--recent progress and persisting challenges.Purinergic signalling · 2012Review
- Purines as potential morphogens during embryonic development.Purinergic signalling · 2012Review
- A mechanism of intracellular P2X receptor activation.The Journal of biological chemistry · 2012Article
- Molecular basis of selective antagonism of the P2X1 receptor for ATP by NF449 and suramin: contribution of basic amino acids in the cysteine-rich loop.British journal of pharmacology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Indicative of cell surface P2X ion channel activation, extracellular ATP evokes a rapid and transient calcium influx in the model eukaryote Dictyostelium discoideum. Five P2X-like proteins (dP2XA-E) are present in this organism. However, their roles in purinergic signaling are unclear, because dP2XA proved to have an intracellular localization on the contractile vacuole where it is thought to be required for osmoregulation. To determine functional properties of the remaining four dP2X-like proteins and to assess their cellular roles, we recorded membrane currents from expressed cloned receptors and generated a quintuple knock-out Dictyostelium strain devoid of dP2X receptors. ATP evoked inward currents at dP2XB and dP2XE receptors but not at dP2XC or dP2XD. beta,gamma-Imido-ATP was more potent than ATP at dP2XB but a weak partial agonist at dP2XE. Currents in dP2XB and dP2XE were strongly inhibited by Na(+) but insensitive to copper and the P2 receptor antagonists pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid and suramin. Unusual for P2X channels, dP2XA and dP2XB were also Cl(-)-permeable. The extracellular purinergic response to ATP persisted in p2xA/B/C/D/E quintuple knock-out Dictyostelium demonstrating that dP2X channels are not responsible. dP2XB, -C, -D, and -E were found to be intracellularly localized to the contractile vacuole with the ligand binding domain facing the lumen. However, quintuple p2xA/B/C/D/E null cells were still capable of regulating cell volume in water demonstrating that, contrary to previous findings, dP2X receptors are not required for osmoregulation. Responses to the calmodulin antagonist calmidazolium, however, were reduced in p2xA/B/C/D/E null cells suggesting that dP2X receptors play a role in intracellular calcium signaling.
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Registered trials
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