Evidence map›Paper›PMID 19832973›Full record

ArticleBMC genomics2009

Development and mapping of DArT markers within the Festuca - Lolium complex.

David Kopecký, Jan Bartos, Adam J Lukaszewski, James H Baird, Vladimír Cernoch, Roland Kölliker, Odd Arne Rognli, Helene Blois, Vanessa Caig, Thomas Lübberstedt and 4 more

Abstract read
In one paragraph

Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

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  12. Development of a molecular linkage map of pearl millet integrating DArT and SSR markers.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011
    Article
  13. Article
  14. High-throughput genotyping of hop (Humulus lupulus L.) utilising diversity arrays technology (DArT).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011
    Article
  15. Genetic mapping of DArT markers in the Festuca-Lolium complex and their use in freezing tolerance association analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011
    Article
  16. Genomic constitution of Festuca × Lolium hybrids revealed by the DArTFest array.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011
    Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

David KopeckýLaboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovská 6, CZ-77200, Olomouc, Czech Republic. kopecky@ueb.cas.cz
Jan Bartos
Adam J Lukaszewski
James H Baird
Vladimír Cernoch
Roland Kölliker
Odd Arne Rognli
Helene Blois
Vanessa Caig
Thomas Lübberstedt
Bruno Studer
Paul Shaw
Jaroslav Dolezel
Andrzej Kilian

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGrasses are among the most important and widely cultivated plants on Earth. They provide high quality fodder for livestock, are used for turf and amenity purposes, and play a fundamental role in environment protection. Among cultivated grasses, species within the Festuca-Lolium complex predominate, especially in temperate regions. To facilitate high-throughput genome profiling and genetic mapping within the complex, we have developed a Diversity Arrays Technology (DArT) array for five grass species: F. pratensis, F. arundinacea, F. glaucescens, L. perenne and L. multiflorum.

resultsThe DArTFest array contains 7680 probes derived from methyl-filtered genomic representations. In a first marker discovery experiment performed on 40 genotypes from each species (with the exception of F. glaucescens for which only 7 genotypes were used), we identified 3884 polymorphic markers. The number of DArT markers identified in every single genotype varied from 821 to 1852. To test the usefulness of DArTFest array for physical mapping, DArT markers were assigned to each of the seven chromosomes of F. pratensis using single chromosome substitution lines while recombinants of F. pratensis chromosome 3 were used to allocate the markers to seven chromosome bins.

conclusionThe resources developed in this project will facilitate the development of genetic maps in Festuca and Lolium, the analysis on genetic diversity, and the monitoring of the genomic constitution of the Festuca x Lolium hybrids. They will also enable marker-assisted selection for multiple traits or for specific genome regions.

Indexed as

Physical Chromosome MappingChromosomes, PlantDNA, PlantFestucaGenetic MarkersGenetic VariationGenotypeLoliumOligonucleotide Array Sequence AnalysisSequence Analysis, DNADNA, PlantGenetic Markers

Identifiers

PMID19832973
PMCPMC2770082

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.