ArticleFunctional & integrative genomics2010
Insulin, a key regulator of hormone responsive milk protein synthesis during lactogenesis in murine mammary explants.
Article in Functional & integrative genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 68 citations in OpenAlex.
- Review
- Causes of Low Milk Supply: The Roles of Estrogens, Progesterone, and Related External Factors.Advances in nutrition (Bethesda, Md.) · 2024Review
- Alterations of milk oligosaccharides in mothers with gestational diabetes mellitus impede colonization of beneficial bacteria and development of RORγtGut microbes · 2023Article
- Protocol for an observational study investigating hormones triggering the onset of sustained lactation: the INSIGHT study.BMJ open · 2022Article
- Developing dietary interventions as therapy for cancer.Nature reviews. Cancer · 2022Review
- Measures of Maternal Metabolic Health as Predictors of Severely Low Milk Production.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2022Article
- Lactational Amenorrhea: Neuroendocrine Pathways Controlling Fertility and Bone Turnover.International journal of molecular sciences · 2022Review
- Human Milk Glucose, Leptin, and Insulin Predict Cessation of Full Breastfeeding and Initiation of Formula Use.Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine · 2021Article
- A Comparative Review of the Extrinsic and Intrinsic Factors Regulating Lactose Synthesis.Journal of mammary gland biology and neoplasia · 2021Review
- Insulin regulates human mammosphere development and function.Cell and tissue research · 2021Article
- Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands.Scientific reports · 2021Article
- Impacts of Heat Stress-Induced Oxidative Stress on the Milk Protein Biosynthesis of Dairy Cows.Animals : an open access journal from MDPI · 2021Review
- Pivotal roles of prolactin and other hormones in lactogenesis and the nutritional composition of human milk.Clinical and experimental pediatrics · 2020Article
- Article
- Regulation of the JAK2-STAT5 Pathway by Signaling Molecules in the Mammary Gland.Frontiers in cell and developmental biology · 2020Review
- Mifepristone Treatment in Pregnant Murine Model Induced Mammary Gland Dysplasia and Postpartum Hypogalactia.Frontiers in cell and developmental biology · 2020Article
- Article
- Genome Wide Identification of Novel Long Non-coding RNAs and Their Potential Associations With Milk Proteins in Chinese Holstein Cows.Frontiers in genetics · 2018Article
- Biological underpinnings of breastfeeding challenges: the role of genetics, diet, and environment on lactation physiology.American journal of physiology. Endocrinology and metabolism · 2016Review
- Does Insulin Explain the Relation between Maternal Obesity and Poor Lactation Outcomes? An Overview of the Literature.Advances in nutrition (Bethesda, Md.) · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine milk protein gene expression requires insulin, hydrocortisone, and prolactin; however, the role of insulin is not well understood. This study, therefore, examined the requirement of insulin for milk protein synthesis. Mammary explants were cultured in various combinations of the lactogenic hormones and global changes in gene expression analysed using Affymetrix microarray. The expression of 164 genes was responsive to insulin, and 18 were involved in protein synthesis at the level of transcription and posttranscription, as well as amino acid uptake and metabolism. The folate receptor gene was increased by fivefold, highlighting a potentially important role for the hormone in folate metabolism, a process that is emerging to be central for protein synthesis. Interestingly, gene expression of two milk protein transcription factors, Stat5a and Elf5, previously identified as key components of prolactin signalling, both showed an essential requirement for insulin. Subsequent experiments in HCll cells confirmed that Stat5a and Elf5 gene expression could be induced in the absence of prolactin but in the presence of insulin. Whereas prolactin plays an essential role in phosphorylating and activating Stat5a, gene expression is only induced when insulin is present. This indicates insulin plays a crucial role in the transcription of the milk protein genes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.