ArticleHistochemistry and cell biology2009
Switching of actin isoforms in skeletal muscle differentiation using mouse ES cells.
Article in Histochemistry and cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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4 citing papers in PubMed, 10 citations in OpenAlex.
- Review
- CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Extending the knowledge in histochemistry and cell biology.Histochemistry and cell biology · 2010Review
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5 authors at 1 institution in 1 country.
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Abstract
Among six actin isoforms, alpha-skeletal and alpha-cardiac actins have similar amino acid components and are highly conserved. Although skeletal muscles essentially express alpha-skeletal actins in the adult tissue, alpha-cardiac isoform actin is prominent in the embryonic muscle tissue. Switching of actin isoforms from alpha-cardiac to alpha-skeletal actin occurs during skeletal muscle differentiation. The cardiac type alpha-actin is expressed in the regeneration and patho-physiological states of the skeletal muscles as well. In the present study, we demonstrate the morphological switching of alpha-type actin isoforms from alpha-cardiac to alpha-skeletal actin in vitro using mouse ES cells for the first time. Immunofluorescent double staining with two specific antibodies revealed that alpha-cardiac actin appeared first in myoblasts. After cell fusion to form myotubes, the cardiac type actin decreased and alpha-skeletal actin conversely increased. Finally, the alpha-skeletal isoform remained as a main actin component in the fully mature skeletal muscle fibers. The exchange of isoforms is not directly linked to the sarcomere formation. As a result, ES cells provide a useful in vitro system for exploring skeletal muscle differentiation.
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