ArticleJournal of clinical microbiology2009
Development and application of a universal Hemoplasma screening assay based on the SYBR green PCR principle.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Molecular Characterization of HemotropicPathogens (Basel, Switzerland) · 2026Article
- First PCR-Confirmed Case of Feline Hemoplasmosis in Bosnia and Herzegovina with a Long-Term Follow-Up.Veterinary sciences · 2026Article
- Ticks and tick-borne bacterial pathogens found on hard ticks (Acari: Ixodidae) on cattle in the Central River region of The Gambia.Medical and veterinary entomology · 2026Article
- Molecular detection of hemotropic mycoplasmas (hemoplasmas) in humans and dogs living on islands and the seashore mainland of Brazil: a One Health approach.Parasites & vectors · 2026Article
- Health profile of captive African pygmy hedgehogs (Atelerix albiventris) in Paraná State, Southern Brazil.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026Article
- Molecular Survey of Hemopathogens in Dogs, Including Blood Donors, from Central-Western Brazil.Pathogens (Basel, Switzerland) · 2025Article
- One Health approach to hemotropic mycoplasmas (hemoplasmas): Molecular detection inOne health (Amsterdam, Netherlands) · 2025Article
- Hemotropic mycoplasmas (hemoplasmas) in indigenous populations and their dogs living in reservation areas, Brazil.Scientific reports · 2025Article
- Molecular detection of hemoplasmas in domestic cats from different Brazilian regions.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2025Article
- Feline Vector-Borne Diseases and Their Possible Association with Hematological Abnormalities in Cats from Midwestern Brazil.Microorganisms · 2024Article
- Vector-borne diseases imported to Poland between 2021 and 2023.Journal of veterinary research · 2024Article
- Molecular detection and identification of hemotropic Mycoplasma species in dogs and their ectoparasites in Iran.Scientific reports · 2024Article
- Molecular Survey of HemotropicPathogens (Basel, Switzerland) · 2023Article
- 'Microorganisms · 2022Article
- Haemoplasma Prevalence and Diversity in Three InvasiveMicroorganisms · 2022Article
- Article
- Review of clinical aspects, epidemiology and diagnosis of haemotropic Mycoplasma ovis in small ruminants: current status and future perspectives in tropics focusing on Malaysia.Tropical animal health and production · 2020Review
- Prevalence and phylogeny of Chlamydiae and hemotropic mycoplasma species in captive and free-living bats.BMC microbiology · 2020Article
- A molecular survey of vector-borne pathogens and haemoplasmas in owned cats across Italy.Parasites & vectors · 2020Article
- A review on the occurrence of companion vector-borne diseases in pet animals in Latin America.Parasites & vectors · 2019Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hemotropic mycoplasmas (hemoplasmas) are the causative agents of infectious anemia in several mammalian species. Their zoonotic potential has recently been substantiated by the identification of a feline hemoplasma isolate in an immunocompromised human patient. Although species-specific diagnostic molecular methods have been developed, their application as screening tools is limited due to the species diversity of hemoplasmas. The goals of this study were to develop a universal hemoplasma screening assay with broad specificity based on the SYBR green PCR principle, to compare the assay with hemoplasma-specific TaqMan PCR, and to analyze potential tick vectors and human blood samples to address the zoonotic potential. The newly developed PCR assay based on the 16S rRNA gene amplified feline, canine, bovine, porcine, camelid, and murine hemoplasmas, as well as Mycoplasma penetrans and Mycoplasma pneumoniae. The lower detection limit for feline and canine hemoplasmas was 1 to 10 copies/PCR. The assay exhibited 98.2% diagnostic sensitivity and 92.1% diagnostic specificity for feline hemoplasmas. All 1,950 Ixodes ticks were PCR negative, suggesting that Ixodes ticks are not relevant vectors for the above-mentioned hemoplasma species in Switzerland. None of the 414 blood samples derived from anemic or immunocompromised human patients revealed a clear positive result. The SYBR green PCR assay described here is a suitable tool to screen for known and so-far-undiscovered hemoplasma species. Positive results should be confirmed by specific TaqMan PCR or sequencing.
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