ArticleJournal of clinical microbiology2009
Diagnostic bioluminescent phage for detection of Yersinia pestis.
Article in Journal of clinical microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 77 citations in OpenAlex.
- Plagued by the Past, Pressed by the Present: A One Health Perspective onBiomedicines · 2025Review
- Multiplex PCR System for the Diagnosis of Plague.Current molecular medicine · 2025Review
- Truncated M13 phage for smart detection of E. coli under dark field.Journal of nanobiotechnology · 2024Article
- Article
- Bacteriophage-Based Biosensors: A Platform for Detection of Foodborne Bacterial Pathogens from Food and Environment.Biosensors · 2022Review
- Lipopolysaccharide of theBiomolecules · 2021Review
- Reporter-Phage-Based Detection and Antibiotic Susceptibility Testing ofMicroorganisms · 2021Article
- Luminescent Phage-Based Detection ofPharmaceuticals (Basel, Switzerland) · 2021Article
- Characterization ofViruses · 2021Article
- Yersinia pestis: the Natural History of Plague.Clinical microbiology reviews · 2020Review
- Review
- Specific Detection ofPathogens (Basel, Switzerland) · 2020Article
- Bacteriophage Based Biosensors: Trends, Outcomes and Challenges.Nanomaterials (Basel, Switzerland) · 2020Review
- Bacteriophages in Natural and Artificial Environments.Pathogens (Basel, Switzerland) · 2019Review
- Engineering of Bacteriophages Y2::Applied and environmental microbiology · 2017Article
- Highly Sensitive Bacteriophage-Based Detection of Brucella abortus in Mixed Culture and Spiked Blood.Viruses · 2017Article
- Substrate-independent luminescent phage-based biosensor to specifically detect enteric bacteria such as E. coli.Environmental science and pollution research international · 2017Article
- Isolation and development of bioluminescent reporter phages for bacterial dysentery.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Rapid detection and simultaneous antibiotic susceptibility analysis of Yersinia pestis directly from clinical specimens by use of reporter phage.Journal of clinical microbiology · 2014Article
- Application of bacteriophages for detection of foodborne pathogens.Bacteriophage · 2014Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Yersinia pestis is the etiological agent of the plague. Because of the disease's inherent communicability, rapid clinical course, and high mortality, it is critical that an outbreak, whether it is natural or deliberate, be detected and diagnosed quickly. The objective of this research was to generate a recombinant luxAB ("light")-tagged reporter phage that can detect Y. pestis by rapidly and specifically conferring a bioluminescent signal response to these cells. The bacterial luxAB reporter genes were integrated into a noncoding region of the CDC plague-diagnostic phage phiA1122 by homologous recombination. The identity and fitness of the recombinant phage were assessed through PCR analysis and lysis assays and functionally verified by the ability to transduce a bioluminescent signal to recipient cells. The reporter phage conferred a bioluminescent phenotype to Y. pestis within 12 min of infection at 28 degrees C. The signal response time and signal strength were dependent on the number of cells present. A positive signal was obtained from 10(2) cells within 60 min. A signal response was not detectable with Escherichia coli, although a weak signal (100-fold lower than that with Y. pestis) was obtained with 1 (of 10) Yersinia enterocolitica strains and 2 (of 10) Yersinia pseudotuberculosis strains at the restrictive temperature. Importantly, serum did not prevent the ability of the reporter phage to infect Y. pestis, nor did it significantly quench the resulting bioluminescent signal. Collectively, the results indicate that the reporter phage displays promise for the rapid and specific diagnostic detection of cultivated Y. pestis isolates or infected clinical specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.