ArticleJournal of virology2010
Microarray analysis of Paramecium bursaria chlorella virus 1 transcription.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Genomics and evolutionary analysis ofJournal of virology · 2024Article
- Early-Phase Drive to the Precursor Pool: Chloroviruses Dive into the Deep End of Nucleotide Metabolism.Viruses · 2023Article
- The Astounding World of Glycans from Giant Viruses.Chemical reviews · 2022Review
- Strong selection and high mutation supply characterize experimental Chlorovirus evolution.Virus evolution · 2022Article
- Comparative Analysis of Transcriptional Regulation Patterns: Understanding the Gene Expression Profile inPathogens (Basel, Switzerland) · 2021Review
- SMRT Sequencing of Paramecium Bursaria Chlorella Virus-1 Reveals Diverse Methylation Stability in Adenines Targeted by Restriction Modification Systems.Frontiers in microbiology · 2020Article
- Chloroviruses.Viruses · 2019Review
- The feedback between selection and demography shapes genomic diversity during coevolution.Science advances · 2019Article
- Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host.PloS one · 2019Article
- Article
- Review
- A comparative review of viral entry and attachment during large and giant dsDNA virus infections.Archives of virology · 2017Review
- Chloroviruses Have a Sweet Tooth.Viruses · 2017Review
- Giant Chloroviruses: Five Easy Questions.PLoS pathogens · 2016Review
- Coevolution mechanisms that adapt viruses to genetic code variations implemented in their hosts.Journal of genetics · 2016Article
- Exploiting tRNAs to Boost Virulence.Life (Basel, Switzerland) · 2016Review
- Chlorovirus PBCV-1 encodes an active copper-zinc superoxide dismutase.Journal of virology · 2014Article
- Deep RNA sequencing reveals hidden features and dynamics of early gene transcription in Paramecium bursaria chlorella virus 1.PloS one · 2014Article
- Viral metagenomics analysis of planktonic viruses in East Lake, Wuhan, China.Virologica Sinica · 2013Article
- Two distinct SSB protein families in nucleo-cytoplasmic large DNA viruses.Bioinformatics (Oxford, England) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Paramecium bursaria chlorella virus 1 (PBCV-1), a member of the family Phycodnaviridae, is a large double-stranded DNA, plaque-forming virus that infects the unicellular green alga Chlorella sp. strain NC64A. The 330-kb PBCV-1 genome is predicted to encode 365 proteins and 11 tRNAs. To monitor global transcription during PBCV-1 replication, a microarray containing 50-mer probes to the PBCV-1 365 protein-encoding genes (CDSs) was constructed. Competitive hybridization experiments were conducted by using cDNAs from poly(A)-containing RNAs obtained from cells at seven time points after virus infection. The results led to the following conclusions: (i) the PBCV-1 replication cycle is temporally programmed and regulated; (ii) 360 (99%) of the arrayed PBCV-1 CDSs were expressed at some time in the virus life cycle in the laboratory; (iii) 227 (62%) of the CDSs were expressed before virus DNA synthesis begins; (iv) these 227 CDSs were grouped into two classes: 127 transcripts disappeared prior to initiation of virus DNA synthesis (considered early), and 100 transcripts were still detected after virus DNA synthesis begins (considered early/late); (v) 133 (36%) of the CDSs were expressed after virus DNA synthesis begins (considered late); and (vi) expression of most late CDSs is inhibited by adding the DNA replication inhibitor, aphidicolin, prior to virus infection. This study provides the first comprehensive evaluation of virus gene expression during the PBCV-1 life cycle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.