ArticleThe Journal of biological chemistry2009
Functional expression of an arachnid sodium channel reveals residues responsible for tetrodotoxin resistance in invertebrate sodium channels.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- Spider venom peptides Ht1a and Gg1a are toxic to honeybee parasitenpj drug discovery · 2026Article
- Tetrodotoxin: The State-of-the-Art Progress in Characterization, Detection, Biosynthesis, and Transport Enrichment.Marine drugs · 2024Review
- In Silico Analysis of Tetrodotoxin Binding in Voltage-Gated Sodium Ion Channels from Toxin-Resistant Animal Lineages.Marine drugs · 2022Article
- Gene Conversion Facilitates the Adaptive Evolution of Self-Resistance in Highly Toxic Newts.Molecular biology and evolution · 2021Article
- Article
- Adaptive venom evolution and toxicity in octopods is driven by extensive novel gene formation, expansion, and loss.GigaScience · 2020Article
- Distinct functional properties of sodium channel variants are associated with usage of alternative exons in Nilaparvata lugens.Insect biochemistry and molecular biology · 2020Article
- Genomic Takeover by Transposable Elements in the Strawberry Poison Frog.Molecular biology and evolution · 2018Article
- Eukaryotic Voltage-Gated Sodium Channels: On Their Origins, Asymmetries, Losses, Diversification and Adaptations.Frontiers in physiology · 2018Article
- Complementary mechanisms for neurotoxin resistance in a copepod.Scientific reports · 2017Article
- Sodium channel diversity in the vestibular ganglion: NaV1.5, NaV1.8, and tetrodotoxin-sensitive currents.Journal of neurophysiology · 2016Article
- Selectivity filters and cysteine-rich extracellular loops in voltage-gated sodium, calcium, and NALCN channels.Frontiers in physiology · 2015Review
- Molecular biology of insect sodium channels and pyrethroid resistance.Insect biochemistry and molecular biology · 2014Article
- A single compartment model of pacemaking in dissasociated substantia nigra neurons: stability and energy analysis.Journal of computational neuroscience · 2013Article
- Diversity and Convergence of Sodium Channel Mutations Involved in Resistance to Pyrethroids.Pesticide biochemistry and physiology · 2013Article
- Constraint shapes convergence in tetrodotoxin-resistant sodium channels of snakes.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Molecular characterization and functional expression of the DSC1 channel.Insect biochemistry and molecular biology · 2011Article
- Genetic architecture of a feeding adaptation: garter snake (Thamnophis) resistance to tetrodotoxin bearing prey.Proceedings. Biological sciences · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Tetrodotoxin (TTX) is a potent blocker of voltage-gated sodium channels, but not all sodium channels are equally sensitive to inhibition by TTX. The molecular basis of differential TTX sensitivity of mammalian sodium channels has been largely elucidated. In contrast, our knowledge about the sensitivity of invertebrate sodium channels to TTX remains poor, in part because of limited success in functional expression of these channels. In this study, we report the functional characterization in Xenopus oocytes of the first non-insect, invertebrate voltage-gated sodium channel from the varroa mite (Varroa destructor), an ecto-parasite of the honeybee. This arachnid sodium channel activates and inactivates rapidly with half-maximal activation at -18 mV and half-maximal fast inactivation at -29 mV. Interestingly, this arachnid channel showed surprising TTX resistance. TTX blocked this channel with an IC(50) of 1 microM. Subsequent site-directed mutagenesis revealed two residues, Thr-1674 and Ser-1967, in the pore-forming region of domains III and IV, respectively, which were responsible for the observed resistance to inhibition by TTX. Furthermore, sequence comparison and additional amino acid substitutions suggested that sequence polymorphisms at these two positions could be a widespread mechanism for modulating TTX sensitivity of sodium channels in diverse invertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.