ArticleProceedings of the National Academy of Sciences of the United States of America2009
Stepwise formation of P-cluster in nitrogenase MoFe protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- High energy resolution fluorescence detected X-ray absorption spectroscopy (HERFD-XAS) for studies of metals and metalloids in biology: current innovations and future perspectives.Metallomics : integrated biometal science · 2025Review
- Stepwise and reversible assembly of [2Fe-2S] rhombs to [8Fe-8S] clusters and their topological interconversions.Nature chemistry · 2025Article
- NifEN: a versatile player in nitrogenase assembly, catalysis and evolution.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2025Review
- Iron-molybdenum cofactor synthesis by a thermophilic nitrogenase devoid of the scaffold NifEN.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ammonia synthesis via an engineered nitrogenase assembly pathway inNature catalysis · 2024Article
- Heterologous synthesis of the complex homometallic cores of nitrogenase P- and M-clusters inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Enzymatic Fischer-Tropsch-Type Reactions.Chemical reviews · 2023Review
- Nitrogenase Fe Protein: A Multi-Tasking Player in Substrate Reduction and Metallocluster Assembly.Molecules (Basel, Switzerland) · 2022Review
- Second and Outer Coordination Sphere Effects in Nitrogenase, Hydrogenase, Formate Dehydrogenase, and CO Dehydrogenase.Chemical reviews · 2022Review
- Biosynthesis of Nitrogenase Cofactors.Chemical reviews · 2020Review
- The Spectroscopy of Nitrogenases.Chemical reviews · 2020Review
- Electron Transfer in Nitrogenase.Chemical reviews · 2020Review
- Chimeric Interaction of Nitrogenase-Like Reductases with the MoFe Protein of Nitrogenase.Chembiochem : a European journal of chemical biology · 2020Article
- Double-Cubane [8Fe9S] Clusters: A Novel Nitrogenase-Related Cofactor in Biology.Chembiochem : a European journal of chemical biology · 2020Review
- The NifZ accessory protein has an equivalent function in maturation of both nitrogenase MoFe protein P-clusters.The Journal of biological chemistry · 2019Article
- A VTVH MCD and EPR Spectroscopic Study of the Maturation of the "Second" Nitrogenase P-Cluster.Inorganic chemistry · 2018Article
- Production and isolation of vanadium nitrogenase from Azotobacter vinelandii by molybdenum depletion.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2017Article
- Negative cooperativity in the nitrogenase Fe protein electron delivery cycle.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Assembly scaffold NifEN: A structural and functional homolog of the nitrogenase catalytic component.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Biosynthesis of nitrogenase metalloclusters.Chemical reviews · 2014Review
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Authors and funding
8 authors.
Funding
Abstract
The P-cluster of nitrogenase is one of the most complex biological metallocenters known to date. Despite the recent advances in the chemical synthesis of P-cluster topologs, the biosynthetic mechanism of P-cluster has not been well defined. Here, we present a combined biochemical, electron paramagnetic resonance, and X-ray absorption spectroscopy/extended X-ray absorption fine-structure investigation of the maturation process of P-clusters in DeltanifH molybdenum-iron (MoFe) protein. Our data indicate that the previously identified, [Fe(4)S(4)]-like cluster pairs in DeltanifH MoFe protein are indeed the precursors to P-clusters, which can be reductively coupled into the mature [Fe(8)S(7)] structures in the presence of Fe protein, MgATP, and dithionite. Moreover, our observation of a biphasic maturation pattern of P-clusters in DeltanifH MoFe protein provides dynamic proof for the previously hypothesized, stepwise assembly mechanism of the two P-clusters in the alpha(2)beta(2)-tetrameric MoFe protein, i.e., one P-cluster is formed in one alphabeta dimer before the other in the second alphabeta dimer.
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