ArticleProceedings of the National Academy of Sciences of the United States of America2009
Myosin VI undergoes a 180 degrees power stroke implying an uncoupling of the front lever arm.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Kinematics of the lever arm swing in myosin VI.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Optimized measurements of separations and angles between intra-molecular fluorescent markers.Nature communications · 2015Article
- Tilting and wobble of myosin V by high-speed single-molecule polarized fluorescence microscopy.Biophysical journal · 2013Article
- Myosin VI has a one track mind versus myosin Va when moving on actin bundles or at an intersection.Traffic (Copenhagen, Denmark) · 2013Article
- Mesoscopic analysis of motion and conformation of cross-bridges.Biophysical reviews · 2012Review
- Processive steps in the reverse direction require uncoupling of the lead head lever arm of myosin VI.Molecular cell · 2012Article
- How myosin motors power cellular functions: an exciting journey from structure to function: based on a lecture delivered at the 34th FEBS Congress in Prague, Czech Republic, July 2009.The FEBS journal · 2012Article
- Structural and functional insights on the Myosin superfamily.Bioinformatics and biology insights · 2012Article
- A conformational transition in the myosin VI converter contributes to the variable step size.Biophysical journal · 2011Article
- Nanogel surface coatings for improved single-molecule imaging substrates.Journal of the Royal Society, Interface · 2011Article
- Lever-arm mechanics of processive myosins.Biophysical journal · 2011Review
- Cargo binding activates myosin VIIA motor function in cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Role of insert-1 of myosin VI in modulating nucleotide affinity.The Journal of biological chemistry · 2011Article
- Nucleotide-dependent shape changes in the reverse direction motor, myosin VI.Biophysical journal · 2010Article
- Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Myosin VI lever arm rotation: fixed or variable?Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Myosin VI: an innovative motor that challenged the swinging lever arm hypothesis.Nature reviews. Molecular cell biology · 2010Review
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Authors and funding
6 authors.
Funding
Abstract
We simultaneously measure both the step size, via FIONA, and the 3-D orientation, via DOPI, of the light-chain domain of individual dimeric myosin VIs. This allows for the correlation of the change in orientation of the light chain domain to the stepping of the motor. Three different pairs of positions were tested using a rigid bifunctional rhodamine on the calmodulin of the IQ domain. The data for all three labeling positions support the model that the light chain domain undergoes a significant rotation of approximately 180 degrees . Contrary to an earlier study [Sun, Y. et al. (2007) Mol Cell 28, 954-964], our data does not support a model of multiple angles of the lever arm of the lead head, nor "wiggly" walking on actin. Instead, we propose that for the two heads of myosin VI to coordinate their processive movement, the lever arm of the lead head must be uncoupled from the converter until the rear head detaches. More specifically, intramolecular strain causes the myosin VI lever arm of the lead head to uncouple from the motor domain, allowing the motor domain to go through its product-release (phosphate and ADP) steps at an unstrained rate. The lever arm of the lead head rebinds to the motor and attains a rigor conformation when the rear head detaches. By coupling the orientation and position information with previously described kinetics, this allows us to explain how myosin VI coordinates its heads processively while maintaining the ability to move under load with a (semi-) rigid lever arm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.