ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2009
Global histone profiling by LC-FTMS after inhibition and knockdown of deacetylases in human cells.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae.Genetics · 2022Article
- Hypoxia and hypoxia mimetics differentially modulate histone post-translational modifications.Epigenetics · 2021Article
- Bullet points to evaluate the performance of the middle-down proteomics workflow for histone modification analysis.Methods (San Diego, Calif.) · 2020Article
- Continuous Elution Proteoform Identification of Myelin Basic Protein by Superficially Porous Reversed-Phase Liquid Chromatography and Fourier Transform Mass Spectrometry.Analytical chemistry · 2017Article
- Applying label-free quantitation to top down proteomics.Analytical chemistry · 2014Article
- Top Down proteomics: facts and perspectives.Biochemical and biophysical research communications · 2014Review
- Oncogene-induced cellular senescence elicits an anti-Warburg effect.Proteomics · 2013Article
- Analysis of Histone Modifications from Tryptic Peptides of Deuteroacetylated Isoforms.International journal of mass spectrometry · 2012Article
- The significance, development and progress of high-throughput combinatorial histone code analysis.Cellular and molecular life sciences : CMLS · 2010Review
- Peptide arrays identify isoform-selective substrates for profiling endogenous lysine deacetylase activity.ACS chemical biology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Global histone modifications and their putative relevance to short and long term cellular programming have drawn substantial interest in the study of chromatin. Here we describe the use of reverse-phase liquid chromatography coupled to Linear Ion Trap-Fourier Transform Mass Spectrometry (RPLC-LTQ-FTMS) to quickly profile post-translationally modified isoforms and variants for core histone proteins from as few as 5x10(4) cells at isotopic resolution. Such LC-MS profiling greatly facilitated the detection of histones from HeLa S3 or 293T cells experiencing shRNA- or siRNA-knockdown of histone deacetylase (HDAC) 1, 2, 3 or 1 and 2 together. In no case was significant global histone hyperacetylation relative to control cells observed, suggesting possible compensation of deacetylation activity by partially redundant enzymes in the 18-member HDAC family. This contrasts sharply with yeast where genetic deletion of HDAC rpd3 causes massive hyperacetylation. Treatment of cells with TSA and class I selective HDAC inhibitors had similar ability to induce global histone hyperactylation, though to different extents in HeLa S3 vs. 293T cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.