ArticleBrain research bulletin2010
Adenovirus-driven overexpression of proteinases in organ-cultured normal human corneas leads to diabetic-like changes.
Article in Brain research bulletin, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 25 citations in OpenAlex.
- Sildenafil increases AAV9 transduction after a systemic administration and enhances AAV9-dystrophin therapeutic effect in mdx mice.Gene therapy · 2024Article
- Regulatory role of miR-146a in corneal epithelial wound healing via its inflammatory targets in human diabetic cornea.The ocular surface · 2022Article
- The role of the PI3K/AKT signalling pathway in the corneal epithelium: recent updates.Cell death & disease · 2022Review
- Gene Therapy in the Anterior Eye Segment.Current gene therapy · 2022Review
- Systemic diseases and the cornea.Experimental eye research · 2021Review
- Ex Vivo Corneal Organ Culture Model for Wound Healing Studies.Journal of visualized experiments : JoVE · 2019Article
- An Update on Corneal Biomechanics and Architecture in Diabetes.Journal of ophthalmology · 2019Review
- Diabetic complications in the cornea.Vision research · 2017Review
- Caveolins and caveolae in ocular physiology and pathophysiology.Progress in retinal and eye research · 2017Review
- Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells.Journal of visualized experiments : JoVE · 2016Article
- Progress in corneal wound healing.Progress in retinal and eye research · 2015Review
- Sensory nerve regeneration after epithelium wounding in normal and diabetic cornea.Expert review of ophthalmology · 2015Article
- Normalization of wound healing and stem cell marker patterns in organ-cultured human diabetic corneas by gene therapy of limbal cells.Experimental eye research · 2014Article
- Differentiation of human limbal-derived induced pluripotent stem cells into limbal-like epithelium.Stem cells translational medicine · 2014Article
- Targeting miR-146a to treat delayed wound healing in human diabetic organ-cultured corneas.PloS one · 2014Article
- Enhanced wound healing, kinase and stem cell marker expression in diabetic organ-cultured human corneas upon MMP-10 and cathepsin F gene silencing.Investigative ophthalmology & visual science · 2013Article
- Effects of diabetes on the eye.Investigative ophthalmology & visual science · 2013Review
- Differentially expressed wound healing-related microRNAs in the human diabetic cornea.PloS one · 2013Article
- Aldose reductase inhibitor counteracts the enhanced expression of matrix metalloproteinase-10 and improves corneal wound healing in galactose-fed rats.Molecular vision · 2013Article
- Impaired epithelial wound healing and EGFR signaling pathways in the corneas of diabetic rats.Investigative ophthalmology & visual science · 2011Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Our previous data suggested the involvement of matrix metalloproteinase-10 (MMP-10) and cathepsin F (CTSF) in the basement membrane and integrin changes occurring in diabetic corneas. These markers were now examined in normal human organ-cultured corneas upon recombinant adenovirus (rAV)-driven transduction of MMP-10 and CTSF genes. Fifteen pairs of normal autopsy human corneas were used. One cornea of each pair was transduced with rAV expressing either CTSF or MMP-10 genes. 1-2 x 10(8) plaque forming units of rAV per cornea were added to cultures for 48 h with or without sildenafil citrate. The fellow cornea of each pair received control rAV with vector alone. After 6-10 days additional incubation without rAV, corneas were analyzed by Western blot or immunohistochemistry, or tested for healing of 5-mm circular epithelial wounds caused by topical application of n-heptanol. Sildenafil significantly increased epithelial transduction efficiency, apparently by stimulation of rAV endocytosis through caveolae. Corneas transduced with CTSF or MMP-10 genes or their combination had increased epithelial immunostaining of respective proteins compared to fellow control corneas. Staining for diabetic markers integrin alpha(3)beta(1), nidogen-1, nidogen-2, and laminin gamma2 chain became weaker and irregular upon proteinase transduction. Expression of phosphorylated Akt was decreased in proteinase-transduced corneas. Joint overexpression of both proteinases led to significantly slower corneal wound healing that became similar to that observed in diabetic corneas. The data suggest that MMP-10 and CTSF may be responsible for abnormal marker patterns and impaired wound healing in diabetic corneas. Inhibition of these proteinases in diabetic corneas may alleviate diabetic keratopathy symptoms.
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