ArticleJournal of biomedical materials research. Part A2010
Lentivirus delivery by adsorption to tissue engineering scaffolds.
Article in Journal of biomedical materials research. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Improving viability and transfection efficiency with human umbilical cord wharton's jelly cells through use of a ROCK inhibitor.Cellular reprogramming · 2014Trial
- Advances in vehicles for in situ delivery: From classical vectors to biologically inspired structures.Synthetic and systems biotechnology · 2026Review
- Advances in viral vector-based delivery systems for gene therapy: a comprehensive review.3 Biotech · 2025Review
- Review
- IL-10 lentivirus-laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury.Biotechnology and bioengineering · 2021Article
- Regulation of adipose tissue inflammation and systemic metabolism in murine obesity by polymer implants loaded with lentiviral vectors encoding human interleukin-4.Biotechnology and bioengineering · 2020Article
- Regeneration of Damaged Tendon-Bone Junctions (Entheses)-TAK1 as a Potential Node Factor.International journal of molecular sciences · 2020Review
- Biomaterial-Guided Gene Delivery for Musculoskeletal Tissue Repair.Tissue engineering. Part B, Reviews · 2017Review
- Heparin-chitosan nanoparticle functionalization of porous poly(ethylene glycol) hydrogels for localized lentivirus delivery of angiogenic factors.Biomaterials · 2014Article
- Scaffold-mediated lentiviral transduction for functional tissue engineering of cartilage.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- A PLG/HAp composite scaffold for lentivirus delivery.Biomaterials · 2013Article
- Nonintegrating knockdown and customized scaffold design enhances human adipose-derived stem cells in skeletal repair.Stem cells (Dayton, Ohio) · 2011Article
- Engineering biomaterial systems to enhance viral vector gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- At the edge of translation - materials to program cells for directed differentiation.Oral diseases · 2011Review
- Hydrogels to modulate lentivirus delivery in vivo from microporous tissue engineering scaffolds.Drug delivery and translational research · 2011Article
- Phosphatidylserine immobilization of lentivirus for localized gene transfer.Biomaterials · 2010Article
- Lentivirus immobilization to nanoparticles for enhanced and localized delivery from hydrogels.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Biomaterial scaffolds capable of localized gene delivery are being investigated for numerous regenerative medicine applications and as model systems for fundamental studies of tissue formation. In this manuscript, we investigate the delivery of lentivirus from a tissue engineering scaffold using a surface immobilization strategy. Poly(lactide-co-glycolide) (PLG) was employed as the biomaterial for delivery, which has been widely used for a number of tissue engineering applications. The virus was immobilized by freezing and subsequent lyophilization of the virus with the scaffold. The presence of sucrose during freezing and lyophilization maintained the activity of the lentivirus, and was similar to an adenovirus control. Collagen and fibronectin were investigated for their ability to enhance surface immobilization. Fibronectin modestly increased binding and transduction of the adenovirus, yet did not significantly impact the lentivirus delivery. Most of the immobilized lentivirus was released from the scaffold within 24 h. In vivo implantation of the scaffolds yielded transgene expression that persisted for at least 4 weeks. These findings indicate the potential for delivering lentivirus from tissue engineering scaffolds using a surface immobilization strategy. To our knowledge, this report is the first to investigate lentivirus delivery from porous tissue engineering scaffolds. Delivery of lentiviral vectors from PLG scaffolds could provide an efficient and versatile gene delivery system for use with in vitro and in vivo models of tissue formation, and ultimately for therapeutic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.