ArticleMolecular biology reports2010
Characterization of the human IRF-3 promoter and its regulation by the transcription factor E2F1.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Transcription factor E2F1 positively regulates interferon regulatory factor 5 expression in non-small cell lung cancer.OncoTargets and therapy · 2019Article
- Up-regulation of IRF-3 expression through GATA-1 acetylation by histone deacetylase inhibitor in lung adenocarcinoma A549 cells.Oncotarget · 2017Article
- Down-regulated GATA-1 up-regulates interferon regulatory factor 3 in lung adenocarcinoma.Scientific reports · 2017Article
- Cloning and characterizing of the murine IRF-3 gene promoter region.Immunologic research · 2016Article
- Persistent prion infection disturbs the function of Oct-1, resulting in the down-regulation of murine interferon regulatory factor-3.Scientific reports · 2014Article
- Upregulation of a spliced variant of human interferon regulatory factor 3 through binding of the transcription factor Sp1 to the promoter.Biomedical reports · 2014Article
- Activation and regulation of interferon-β in immune responses.Immunologic research · 2012Review
- Characterization of a spliced variant of human IRF-3 promoter and its regulation by the transcription factor Sp1.Molecular biology reports · 2012Article
- Article
- Galectin-9 protein expression in endothelial cells is positively regulated by histone deacetylase 3.The Journal of biological chemistry · 2011Article
- The characterization of two novel IRF-3 transcripts starting from intron 2 of the wild type of IRF-3.Molecular biology reports · 2011Article
- Direct repression of the human IRF-3 promoter by E2F1.Immunogenetics · 2011Article
- Phosphoryl moieties of lipid A from Neisseria meningitidis and N. gonorrhoeae lipooligosaccharides play an important role in activation of both MyD88- and TRIF-dependent TLR4-MD-2 signaling pathways.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interferon regulatory factor 3 (IRF-3), an essential transcriptional regulator of the interferon genes, has been implicated in virus and double-stranded RNA mediated induction of IFN-α, IFN-β and RANTES, in virus-induced apoptosis and in tumor suppression. Promoter plays an important role in the regulation of gene expression, but the characterization of the human IRF-3 promoter has not been systematically analyzed in HEK 293 cells. To characterize the human IRF-3 promoter, we have isolated a genomic clone of the human IRF-3 gene promoter region containing 1,000 nucleotides of the 5'- flanking region. Transient transfection of 5'-deleted promoter-reporter constructs and luciferase assay illustrated the region -149/-93 relative to the transcription start site (TSS) is sufficient for full promoter activity. This region contains HSF, E2F, CdxA and c-Myb transcription factor binding sites. The E2F sites are highly conserved among IRF-3 promoter regions of mouse, rat and human. Therefore, it was suggested that this E2F site may be essential for basal promoter activity. Surprisingly, mutation of this E2F site increased the promoter activity by 2-fold. Furthermore, overexpression of E2F1 reduced the transcription activity by 80%. These results indicated that human IRF-3 gene core promoter was located within the region -149/-93 relative to the TSS. E2F1 transcription factor negatively regulates human IRF-3 gene promoter.
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