ArticleIn vitro cellular & developmental biology. Animal2010
Osteogenesis in vitro: from pre-osteoblasts to osteocytes: a contribution from the Osteobiology Research Group, The Pennsylvania State University.
Article in In vitro cellular & developmental biology. Animal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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Who cites it
10 citing papers in PubMed, 32 citations in OpenAlex.
- Preparation of functional poly-(lactic acid-co-glycolic acid)-based guided bone-regeneration membrane and its application in the reconstruction of mandibular defects in rats.Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2022Article
- Review
- Review
- Osteogenic and Angiogenic Profiles of Mandibular Bone-Forming Cells.Frontiers in physiology · 2019Article
- Review
- Microbeads-Guided Reconstruction of 3D Osteocyte Network during Microfluidic Perfusion Culture.Journal of materials chemistry. B · 2015Article
- Biomechanical forces in the skeleton and their relevance to bone metastasis: biology and engineering considerations.Advanced drug delivery reviews · 2014Review
- Application of retinoic acid to obtain osteocytes cultures from primary mouse osteoblasts.Journal of visualized experiments : JoVE · 2014Article
- A new method to investigate how mechanical loading of osteocytes controls osteoblasts.Frontiers in endocrinology · 2014Article
- Osteocyte differentiation is regulated by extracellular matrix stiffness and intercellular separation.Journal of the mechanical behavior of biomedical materials · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Murine calvariae pre-osteoblasts (MC3T3-E1), grown in a novel bioreactor, proliferate into a mineralizing 3D osteoblastic tissue that undergoes progressive phenotypic maturation into osteocyte-like cells. Initially, the cells are closely packed (high cell/matrix ratio), but transform into a more mature phenotype (low cell/matrix ratio) after about 5 mo, a process that recapitulates stages of bone development observed in vivo. The cell morphology concomitantly evolves from spindle-shaped pre-osteoblasts through cobblestone-shaped osteoblasts to stellate-shaped osteocyte-like cells interconnected by many intercellular processes. Gene-expression profiles parallel cell morphological changes, up-to-and-including increased expression of osteocyte-associated genes such as E11, DMP1, and sclerostin. X-ray scattering and infrared spectroscopy of contiguous, square centimeter-scale macroscopic mineral deposits are consistent with bone hydroxyapatite, showing that bioreactor conditions can lead to ossification reminiscent of bone formation. Thus, extended-term osteoblast culture (< or =10 mo) in a bioreactor based on the concept of simultaneous growth and dialysis captures the full continuum of bone development otherwise inaccessible with conventional cell culture, resulting in an in vitro model of osteogenesis and a source of terminally differentiated osteocytes that does not require demineralization of fully formed bone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.