ArticleCurrent microbiology2010
Evaluation of the expression and protective potential of Leptospiral sphingomyelinases.
Article in Current microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Leptospiral hemolysins induce proinflammatory cytokines through Toll-like receptor 2-and 4-mediated JNK and NF-κB signaling pathways.PloS one · 2012Trial
- Identification of virulence genes and clade-specific markers through pan-genomic analysis of Leptospira.BMC microbiology · 2025Article
- A clinical pilot study for the detection of sphingomyelinase in leptospirosis patient's urine at tertiary care hospital.Heliyon · 2023Article
- Sph2Vaccines · 2023Article
- Extracellular Proteome Analysis Shows the Abundance of Histidine Kinase Sensor Protein, DNA Helicase, Putative Lipoprotein Containing Peptidase M75 Domain and Peptidase C39 Domain Protein inPathogens (Basel, Switzerland) · 2021Article
- Article
- Micronutrients and Leptospirosis: A Review of the Current Evidence.PLoS neglected tropical diseases · 2016Review
- Generation of Mammalian Host-adaptedBio-protocol · 2015Article
- Role of sph2 Gene Regulation in Hemolytic and Sphingomyelinase Activities Produced by Leptospira interrogans.PLoS neglected tropical diseases · 2015Article
- A model system for studying the transcriptomic and physiological changes associated with mammalian host-adaptation by Leptospira interrogans serovar Copenhageni.PLoS pathogens · 2014Article
- Leptospiral outer membrane protein microarray, a novel approach to identification of host ligand-binding proteins.Journal of bacteriology · 2012Article
- Multiple leptospiral sphingomyelinases (or are there?).Microbiology (Reading, England) · 2012Review
- InvA protein is a Nudix hydrolase required for infection by pathogenic Leptospira in cell lines and animals.The Journal of biological chemistry · 2011Article
- Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses.Future microbiology · 2010Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is a zoonotic disease of global distribution, which affects both animals and humans. Pathogenic leptospires, the bacteria that cause this disease, require iron for their growth, and these spirochetes probably use their hemolysins, such as the sphingomyelinases, as a way to obtain this important nutrient from host red blood cells during infection. We expressed and purified the leptospiral sphingomyelinases Sph1, Sph2, Sph4, and SphH in a heterologous system. However, the recombinant proteins were not able to lyse sheep erythrocytes, despite having regular secondary structures. Transcripts for all sphingomyelinases tested were detected by RT-PCR analyses, but only Sph2 and SphH native proteins could be detected in Western blot assays using Leptospira whole extracts as well as in renal tubules of infected hamsters. Moreover, antibodies present in the serum of a human patient with laboratory-confirmed leptospirosis recognized Sph2, indicating that this sphingomyelinase is expressed and exposed to the immune system during infection in humans. However, in an animal challenge model, none of the sphingomyelinases tested conferred protection against leptospirosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.