ArticleApplied microbiology and biotechnology2010
Silencing of Vlaro2 for chorismate synthase revealed that the phytopathogen Verticillium longisporum induces the cross-pathway control in the xylem.
Article in Applied microbiology and biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 68 citations in OpenAlex.
- The Aromatic Amino Acid Biosynthesis GeneMicroorganisms · 2025Article
- Combined Transcriptome and Metabolome Analysis Reveals That Carbon Catabolite Repression Governs Growth and Pathogenicity inInternational journal of molecular sciences · 2024Article
- Nitrate transporter protein NPF5.12 and major latex-like protein MLP6 are important defense factors against Verticillium longisporum.Journal of experimental botany · 2024Article
- The Frq-Frh Complex Light-Dependently Delays Sfl1-Induced Microsclerotia Formation inJournal of fungi (Basel, Switzerland) · 2023Article
- Verticillium dahliae Vta3 promotes ELV1 virulence factor gene expression in xylem sap, but tames Mtf1-mediated late stages of fungus-plant interactions and microsclerotia formation.PLoS pathogens · 2023Article
- Tomato Xylem Sap Hydrophobins Vdh4 and Vdh5 Are Important for Late Stages ofJournal of fungi (Basel, Switzerland) · 2022Article
- A 20-kb lineage-specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum.Molecular plant pathology · 2021Article
- Molecular targets for antifungals in amino acid and protein biosynthetic pathways.Amino acids · 2021Review
- In-depth evaluation of root infection systems using the vascular fungus Verticillium longisporum as soil-borne model pathogen.Plant methods · 2021Article
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Transcriptome Variations inFrontiers in microbiology · 2021Article
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- The Transcriptome ofGenes · 2019Article
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- The oligosaccharyl transferase subunit STT3 mediates fungal development and is required for virulence in Verticillium dahliae.Current genetics · 2018Article
- Rhamnose synthase activity is required for pathogenicity of the vascular wilt fungus Verticillium dahliae.Molecular plant pathology · 2017Article
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- Verticillium longisporum, the invisible threat to oilseed rape and other brassicaceous plant hosts.Molecular plant pathology · 2016Review
- Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae.BMC biotechnology · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum (VL) has been generated. VL enters its host Brassica napus through the roots and colonizes the xylem vessels. The xylem contains little nutrients including low concentrations of amino acids. We isolated the gene Vlaro2 encoding chorismate synthase by complementation of the corresponding yeast mutant strain. Chorismate synthase produces the first branch point intermediate of aromatic amino acid biosynthesis. A novel RNA-mediated gene silencing method reduced gene expression of both isogenes by 80% and resulted in a bradytrophic mutant, which is a leaky auxotroph due to impaired expression of chorismate synthase. In contrast to the wild type, silencing resulted in increased expression of the cross-pathway regulatory gene VlcpcA (similar to cpcA/GCN4) during saprotrophic life. The mutant fungus is still able to infect the host plant B. napus and the model Arabidopsis thaliana with reduced efficiency. VlcpcA expression is increased in planta in the mutant and the wild-type fungus. We assume that xylem colonization requires induction of the cross-pathway control, presumably because the fungus has to overcome imbalanced amino acid supply in the xylem.
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