ArticlePloS one2009
Sumoylation of transcription factor Tec1 regulates signaling of mitogen-activated protein kinase pathways in yeast.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Conserved signaling modules regulate filamentous growth in fungi: a model for eukaryotic cell differentiation.Genetics · 2024Review
- Ecological inducers of the yeast filamentous growth pathway reveal environment-dependent roles for pathway components.mSphere · 2023Article
- Down-regulation of UBC9 increases the sensitivity of hepatocellular carcinoma to doxorubicin.Oncotarget · 2017Article
- Article
- Regulating global sumoylation by a MAP kinase Hog1 and its potential role in osmo-tolerance in yeast.PloS one · 2014Article
- Dynamic ubiquitination of the mitogen-activated protein kinase kinase (MAPKK) Ste7 determines mitogen-activated protein kinase (MAPK) specificity.The Journal of biological chemistry · 2013Article
- Proper protein glycosylation promotes mitogen-activated protein kinase signal fidelity.Biochemistry · 2013Article
- Response to hyperosmotic stress.Genetics · 2012Review
- Combined computational and experimental analysis reveals mitogen-activated protein kinase-mediated feedback phosphorylation as a mechanism for signaling specificity.Molecular biology of the cell · 2012Article
- The evolution and origin of animal Toll-like receptor signaling pathway revealed by network-level molecular evolutionary analyses.PloS one · 2012Article
- The regulation of filamentous growth in yeast.Genetics · 2012Review
- Pheromone- and RSP5-dependent ubiquitination of the G protein beta subunit Ste4 in yeast.The Journal of biological chemistry · 2011Article
- Single-cell analysis reveals that insulation maintains signaling specificity between two yeast MAPK pathways with common components.Science signaling · 2010Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tec1 is a transcription factor in the yeast mitogen-activated protein kinase (MAPK) pathway that controls invasive growth. Previously we reported that a fraction of Tec1 protein is sumoylated on residue lysine 54 in normally growing cells. Here we describe regulation and functional consequences of Tec1 sumoylation. We found that activation of Kss1, the MAPK that directly activates Tec1, results in a decrease in Tec1 sumoylation and a concurrent increase of Tec1 transcriptional activity. Consistent with a role of sumoylation in inhibiting Tec1 activity, specifically increasing sumoylation of Tec1 by fusing it to the sumoylating enzyme Ubc9 leads to a dramatic decrease of Tec1 transcriptional activity. Invasive growth is also compromised in Tec1-Ubc9. In contrast, fusing sumoylation-site mutant Tec1, i.e., Tec1(K54R), to Ubc9 did not significantly alter transcriptional activation and had a less effect on invasive growth. Taken together, these findings provide evidence for regulated sumoylation as a mechanism to modulate the activity of Tec1 and validate Ubc9 fusion-directed sumoylation as a useful approach for studying protein sumoylation.
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