ArticleProceedings of the National Academy of Sciences of the United States of America2009
Kinetics of hyperpolarized 13C1-pyruvate transport and metabolism in living human breast cancer cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
125 citing papers in PubMed, 224 citations in OpenAlex.
- Biocompatible SABRE Hyperpolarization of [1-Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Quantitative Imaging of Pyruvate Metabolism in a Patient With Anaplastic Thyroid Cancer.Magnetic resonance in medicine · 2026Article
- Physiological Interpretation of the Lactate to Pyruvate AUC Ratio for Hyperpolarized [1-Magnetic resonance in medicine · 2026Article
- Robust Rapid Cellular Metabolite Sensing Using Benchtop NMR and SABRE-Hyperpolarized [1-Analytical chemistry · 2026Article
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- Lab-on-a-Chip Metabolic Analysis Using Benchtop NMR Technology.Analytical chemistry · 2026Article
- Review
- Lactylation in cancer biology: Unlocking new avenues for research and therapy.Cancer communications (London, England) · 2025Review
- Lactylation in cancer: Advances and opportunities for treatment resistance.Clinical and translational medicine · 2025Review
- Scalable Hyperpolarized MRI Enabled by Ace-SABRE of [1-Angewandte Chemie (International ed. in English) · 2025Article
- A flexible MRF approach to improve kinetic rate estimation with bSSFP-based hyperpolarized [1-Magnetic resonance in medicine · 2025Article
- Multinuclear MRI Can Depict Metabolic and Energetic Changes in Mild Traumatic Brain Injury.NMR in biomedicine · 2025Article
- Article
- Short-lived calcium carbonate precursors observed in situ via Bullet-dynamic nuclear polarization.Communications chemistry · 2024Article
- Metabolic imaging across scales reveals distinct prostate cancer phenotypes.Nature communications · 2024Article
- Molecular imaging of tumor metabolism: Insight from pyruvate- and glucose-based deuterium MRI studies.Science advances · 2024Article
- A Simulation of the Effects of Diffusion on Hyperpolarized [1-IEEE transactions on bio-medical engineering · 2023Article
- Hyperpolarized Carbon 13 MRI: Clinical Applications and Future Directions in Oncology.Radiology. Imaging cancer · 2023Review
- Hyperpolarized Carbon-13 MRI in Breast Cancer.Diagnostics (Basel, Switzerland) · 2023Review
- Enhancing Cancer Diagnosis with Real-Time Feedback: Tumor Metabolism through Hyperpolarized 1-Metabolites · 2023Review
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Metabolic fluxes can serve as specific biomarkers for detecting malignant transformations, tumor progression, and response to microenvironmental changes and treatment procedures. We present noninvasive hyperpolarized (13)C NMR investigations on the metabolic flux of pyruvate to lactate, in a well-controlled injection/perfusion system using T47D human breast cancer cells. Initial rates of pyruvate-to-lactate conversion were obtained by fitting the hyperpolarized (13)C and ancillary (31)P NMR data to a model, yielding both kinetic parameters and mechanistic insight into this conversion. Transport was found to be the rate-limiting process for the conversion of extracellular pyruvate to lactate with K(m) = 2.14 +/- 0.03 mM, typical of the monocarboxylate transporter 1 (MCT1), and a V(max) = 27.6 +/- 1.1 fmolxmin(-1).cell(-1), in agreement with the high expression level of this transporter. Modulation of the environment to hypoxic conditions as well as suppression of cells' perfusion enhanced the rate of pyruvate-to-lactate conversion, presumably by up-regulation of the MCT1. Conversely, the addition of quercetin, a flavonoidal MCT1 inhibitor, markedly reduces the apparent rate of pyruvate-to-lactate conversion. These results suggest that hyperpolarized (13)C(1)-pyruvate may be a useful magnetic resonance biomarker of MCT regulation and malignant transformations in breast cancer.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.