ArticleNucleic acids research2009
The Crc global regulator binds to an unpaired A-rich motif at the Pseudomonas putida alkS mRNA coding sequence and inhibits translation initiation.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 102 citations in OpenAlex.
- The Catabolite Repression Control Protein Crc Regulates the Type III Secretion System Through the Adenylate Cyclase CyaB inMicroorganisms · 2025Article
- Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds.Microbial biotechnology · 2024Article
- Sphingosine induction of theJournal of bacteriology · 2024Article
- Pseudomonas putida as a platform for medium-chain length α,ω-diol production: Opportunities and challenges.Microbial biotechnology · 2024Article
- GenoMine: a CRISPR-Cas9-based kill switch for biocontainment ofFrontiers in bioengineering and biotechnology · 2024Article
- Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization inApplied and environmental microbiology · 2023Article
- Regulation of hierarchical carbon substrate utilization, nitrogen fixation, and root colonization by the Hfq/Crc/CrcZY genes iniScience · 2022Article
- Pseudomonas syringae addresses distinct environmental challenges during plant infection through the coordinated deployment of polysaccharides.Journal of experimental botany · 2022Article
- Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4.PloS one · 2022Article
- When metabolic prowess is too much of a good thing: how carbon catabolite repression and metabolic versatility impede production of esterified α,ω-diols in Pseudomonas putida KT2440.Biotechnology for biofuels · 2021Article
- The global regulator Hfq exhibits far more extensive and intensive regulation than Crc in Pseudomonas protegens H78.Molecular plant pathology · 2021Article
- Role of a local transcription factor in governing cellular carbon/nitrogen homeostasis in Pseudomonas fluorescens.Nucleic acids research · 2021Article
- Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida.Scientific reports · 2019Article
- Pseudomonas donghuensis HYS virulence towards Caenorhabditis elegans is regulated by the Cbr/Crc system.Scientific reports · 2019Article
- Multi-omics analysis unravels a segregated metabolic flux network that tunes co-utilization of sugar and aromatic carbons inThe Journal of biological chemistry · 2019Article
- High-Yield Production of 4-Hydroxybenzoate From Glucose or Glycerol by an EngineeredFrontiers in bioengineering and biotechnology · 2019Article
- Eliminating a global regulator of carbon catabolite repression enhances the conversion of aromatic lignin monomers to muconate inMetabolic engineering communications · 2017Article
- A metabolic pathway for catabolizing levulinic acid in bacteria.Nature microbiology · 2017Article
- Transcriptional Modulation of Transport- and Metabolism-Associated Gene Clusters Leading to Utilization of Benzoate in Preference to Glucose in Pseudomonas putida CSV86.Applied and environmental microbiology · 2017Article
- Glucose uptake in Azotobacter vinelandii occurs through a GluP transporter that is under the control of the CbrA/CbrB and Hfq-Crc systems.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crc is a key global translational regulator in Pseudomonads that orchestrates the hierarchy of induction of several catabolic pathways for amino acids, sugars, hydrocarbons or aromatic compounds. In the presence of amino acids, which are preferred carbon sources, Crc inhibits translation of the Pseudomonas putida alkS and benR mRNAs, which code for transcriptional regulators of genes required to assimilate alkanes (hydrocarbons) and benzoate (an aromatic compound), respectively. Crc binds to the 5'-end of these mRNAs, but the sequence and/or structure recognized, and the way in which it inhibits translation, were unknown. We have determined the secondary structure of the alkS mRNA 5'-end through its sensitivity to several ribonucleases and chemical reagents. Footprinting and band-shift assays using variant alkS mRNAs have shown that Crc specifically binds to a short unpaired A-rich sequence located adjacent to the alkS AUG start codon. This interaction is stable enough to prevent formation of the translational initiation complex. A similar Crc-binding site was localized at benR mRNA, upstream of the Shine-Dalgarno sequence. This allowed predicting binding sites at other Crc-regulated genes, deriving a consensus sequence that will help to validate new Crc targets and to discriminate between direct and indirect effects of this regulator.
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