ArticleJournal of cell science2009
The transmembrane protein CBP plays a role in transiently anchoring small clusters of Thy-1, a GPI-anchored protein, to the cytoskeleton.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 52 citations in OpenAlex.
- Review
- Review
- Thy-1 as an Integrator of Diverse Extracellular Signals.Frontiers in cell and developmental biology · 2019Review
- Neuronal Signaling by Thy-1 in Nanodomains With Specific Ganglioside Composition: Shall We Open the Door to a New Complexity?Frontiers in cell and developmental biology · 2019Article
- Thy-1/CD90 a Bidirectional and Lateral Signaling Scaffold.Frontiers in cell and developmental biology · 2019Review
- Article
- Thy-1, a Pathfinder Protein for the Post-genomic Era.Frontiers in cell and developmental biology · 2018Review
- Roles for lipid heterogeneity in immunoreceptor signaling.Biochimica et biophysica acta · 2016Review
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Reciprocal Interactions between Cell Adhesion Molecules of the Immunoglobulin Superfamily and the Cytoskeleton in Neurons.Frontiers in cell and developmental biology · 2016Review
- Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction.The Journal of cell biology · 2015Article
- Fixation strategies for retinal immunohistochemistry.Progress in retinal and eye research · 2015Review
- Cytoskeletal confinement of CX3CL1 limits its susceptibility to proteolytic cleavage by ADAM10.Molecular biology of the cell · 2014Article
- Nanoclustering as a dominant feature of plasma membrane organization.Journal of cell science · 2014Review
- PAG--a multipurpose transmembrane adaptor protein.Oncogene · 2014Review
- Regulation from within: the cytoskeleton in transmembrane signaling.Trends in cell biology · 2012Review
- Membranes: first steps to rafts?Nature chemical biology · 2012Article
- Mechanisms underlying the confined diffusion of cholera toxin B-subunit in intact cell membranes.PloS one · 2012Article
- Astrocytic αVβ3 integrin inhibits neurite outgrowth and promotes retraction of neuronal processes by clustering Thy-1.PloS one · 2012Article
- Raft protein clustering alters N-Ras membrane interactions and activation pattern.Molecular and cellular biology · 2011Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
It remains unclear how GPI-anchored proteins (GPIAPs), which lack cytoplasmic domains, transduce signals triggered by specific ligation. Such signal transduction has been speculated to require the ligated GPIAP to associate with membrane-spanning proteins that communicate with obligate cytoplasmic proteins. Transient anchorage of crosslinked proteins on the cell surface was previously characterized by single-particle tracking, and temporary association with the actin cytoskeleton was hypothesized to cause regulated anchorage. GPIAPs, such as Thy-1, require clustering, cholesterol and Src-family kinase (SFK) activity to become transiently anchored. By contrast, a transmembrane protein, the cystic fibrosis transmembrane conductance regulator (CFTR), which has a PDZ-binding motif in its cytoplasmic C-terminus that binds the ERM adaptor EBP50, exhibits anchorage that strictly requires EBP50 but has little dependence on cholesterol or SFK. We hypothesized that a transmembrane protein would be required to mediate the linkage between Thy-1 and the cytoskeleton. Here, we present evidence, obtained by shRNA knockdown, that the transmembrane protein Csk-binding protein (CBP) plays an obligatory role in the transient anchorage of Thy1. Furthermore, either a dominant-negative form of CBP that did not bind EBP50 or a dominant-negative EBP50 drastically reduced transient anchorage of Thy-1, indicating the involvement of this adaptor. Finally, we speculate on the role of phosphorylation in the regulation of transient anchorage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.