ArticleMolecular biology reports2010
A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 29 citations in OpenAlex.
- Effects of DCA gene overexpression in transgenic Dunaliella parva.Antonie van Leeuwenhoek · 2024Article
- Comparative morphological, physiological, biochemical and genomic studies reveal novel genes of Dunaliella bioculata and D. quartolecta in response to salt stress.Molecular biology reports · 2022Article
- OXS2 is Required for Salt Tolerance Mainly through Associating with Salt Inducible Genes, CA1 and Araport11, in Arabidopsis.Scientific reports · 2019Article
- Hydrogen Sulfide-Mediated Activation ofInternational journal of molecular sciences · 2018Article
- The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins.World journal of microbiology & biotechnology · 2014Review
- Hypoosmotic expression of Dunaliella bardawil ζ-carotene desaturase is attributed to a hypoosmolarity-responsive element different from other key carotenogenic genes.Plant physiology · 2014Article
- Article
- Application of synthetic biology in cyanobacteria and algae.Frontiers in microbiology · 2012Article
- The characterization of transaldolase gene tal from Pichia stipitis and its heterologous expression in Fusarium oxysporum.Molecular biology reports · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D. salina are salt-inducible. In this study, the results of real time RT-PCR showed that the transcripts of DCA1 were induced by gradient concentration of sodium chloride. Subsequently, a structurally novel promoter containing highly repeated GT/AC sequences of the DCA1 gene was isolated, which was able to drive a stable expression of the foreign bar gene in transformed cells of D. salina, and the gradient concentrations of sodium chloride in media paralleled regulations in the levels of both proteins and mRNA of the bar gene driven by the DCA1 promoter. Furthermore, analysis of GUS activities revealed that the salt-inducible expression of the external gus gene was regulated by the promoter fragments containing highly repeated GT sequences, but not by the promoter fragments deleting highly repeated GT sequences. The findings above-mentioned suggest that the highly repeated GT sequence in the DCA1 promoter is involved in the salt-inducible regulation in D. salina and may be a novel salt-inducible element.
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