ArticlePloS one2009
Cyanobacterial cell lineage analysis of the spatiotemporal hetR expression profile during heterocyst pattern formation in Anabaena sp. PCC 7120.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
- Spatio-temporal coherence of circadian clocks and temporal control of differentiation inmSystems · 2024Article
- Analysis of fate determination of vegetative cells with reduced phycocyanin content in a multicellular cyanobacterium using Raman microscopy.microPublication biology · 2024Article
- Raman spectral analysis of microbial pigment compositions in vegetative cells and heterocysts of multicellular cyanobacterium.Biochemistry and biophysics reports · 2023Article
- Position-dependent changes in phycobilisome abundance in multicellular cyanobacterial filaments revealed by Raman spectral analysis.microPublication biology · 2023Article
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumFrontiers in microbiology · 2020Article
- cyAbrB Transcriptional Regulators as Safety Devices To Inhibit Heterocyst Differentiation inJournal of bacteriology · 2019Article
- The Ser/Thr Kinase PknH Is Essential for Maintaining Heterocyst Pattern in the CyanobacteriumLife (Basel, Switzerland) · 2018Article
- Soft X-Ray Imaging of Cellular Carbon and Nitrogen Distributions in Heterocystous Cyanobacteria.Plant physiology · 2018Article
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Live-cell imaging of cyanobacteria.Photosynthesis research · 2015Review
- Spatial fluctuations in expression of the heterocyst differentiation regulatory gene hetR in Anabaena filaments.PLoS genetics · 2015Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diazotrophic heterocyst formation in the filamentous cyanobacterium, Anabaena sp. PCC 7120, is one of the simplest pattern formations known to occur in cell differentiation. Most previous studies on heterocyst patterning were based on statistical analysis using cells collected or observed at different times from a liquid culture, which would mask stochastic fluctuations affecting the process of pattern formation dynamics in a single bacterial filament. In order to analyze the spatiotemporal dynamics of heterocyst formation at the single filament level, here we developed a culture system to monitor simultaneously bacterial development, gene expression, and phycobilisome fluorescence. We also developed micro-liquid chamber arrays to analyze multiple Anabaena filaments at the same time. Cell lineage analyses demonstrated that the initial distributions of hetR::gfp and phycobilisome fluorescence signals at nitrogen step-down were not correlated with the resulting distribution of developed heterocysts. Time-lapse observations also revealed a dynamic hetR expression profile at the single-filament level, including transient upregulation accompanying cell division, which did not always lead to heterocyst development. In addition, some cells differentiated into heterocysts without cell division after nitrogen step-down, suggesting that cell division in the mother cells is not an essential requirement for heterocyst differentiation.
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