ArticleThe Journal of biological chemistry2009
Calcium bridge triggers capsid disassembly in the cell entry process of simian virus 40.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Ion Channels as Therapeutic Targets for Viral Infections: Further Discoveries and Future Perspectives.Viruses · 2020Review
- pH stability and disassembly mechanism of wild-type simian virus 40.Soft matter · 2020Article
- Article
- Article
- Systematic analysis of biological roles of charged amino acid residues located throughout the structured inner wall of a virus capsid.Scientific reports · 2018Article
- Principles of Virus Uncoating: Cues and the Snooker Ball.Traffic (Copenhagen, Denmark) · 2016Review
- Chemically activatable viral capsid functionalized for cancer targeting.Nanomedicine (London, England) · 2016Article
- SV40 VP1 major capsid protein in its self-assembled form allows VP1 pentamers to coat various types of artificial beadsBiotechnology reports (Amsterdam, Netherlands) · 2015Article
- The human alpha defensin HD5 neutralizes JC polyomavirus infection by reducing endoplasmic reticulum traffic and stabilizing the viral capsid.Journal of virology · 2014Article
- Quantum dot-induced viral capsid assembling in dissociation buffer.International journal of nanomedicine · 2013Article
- The Simian virus 40 late viral protein VP4 disrupts the nuclear envelope for viral release.Journal of virology · 2012Article
- VP-1 quasispecies in human infection with polyomavirus BK.Journal of medical virology · 2012Article
- Human papillomavirus type 18 chimeras containing the L2/L1 capsid genes from evolutionarily diverse papillomavirus types generate infectious virus.Virus research · 2011Article
- Quaternary structures of HIV Env immunogen exhibit conformational vicissitudes and interface diminution elicited by ligand binding.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Structural evidence of glycoprotein assembly in cellular membrane compartments prior to Alphavirus budding.Journal of virology · 2010Article
- High-resolution x-ray structure and functional analysis of the murine norovirus 1 capsid protein protruding domain.Journal of virology · 2010Article
- Structure and function of a genetically engineered mimic of a nonenveloped virus entry intermediate.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The calcium bridge between the pentamers of polyoma viruses maintains capsid metastability. It has been shown that viral infection is profoundly inhibited by the substitution of lysine for glutamate in one calcium-binding residue of the SV40 capsid protein, VP1. However, it is unclear how the calcium bridge affects SV40 infectivity. In this in vitro study, we analyzed the influence of host cell components on SV40 capsid stability. We used an SV40 mutant capsid (E330K) in which lysine had been substituted for glutamate 330 in protein VP1. The mutant capsid retained the ability to interact with the SV40 cellular receptor GM1, and the internalized mutant capsid accumulated in caveolin-1-mediated endocytic vesicles and was then translocated to the endoplasmic reticulum (ER) region. However, when placed in ER-rich microsome, the mutant capsid retained its spherical structure in contrast to the wild type, which disassembled. Structural analysis of the mutant capsid with cryo-electron microscopy and image reconstruction revealed altered pentamer coordination, possibly as a result of electrostatic interaction, although its overall structure resembled that of the wild type. These results indicate that the calcium ion serves as a trigger at the pentamer interface, which switches on capsid disassembly, and that the failure of the E330K mutant capsid to disassemble is attributable to an inadequate triggering system. Our data also indicate that calcium depletion-induced SV40 capsid disassembly may occur in the ER region and that this is essential for successful SV40 infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.