ArticleBioconjugate chemistry2009
Thiol-reactive derivatives of the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore: a highly sensitive toolset for the detection of biomolecular interactions.
Article in Bioconjugate chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- An evolved artificial radical cyclase enables the construction of bicyclic terpenoid scaffolds via an H-atom transfer pathway.Nature chemistry · 2024Article
- Fluorescent Probes and Quenchers in Studies of Protein Folding and Protein-Lipid Interactions.Chemical record (New York, N.Y.) · 2024Review
- Multicomponent synthesis of chromophores - The one-pot approach to functional π-systems.Frontiers in chemistry · 2023Review
- Polarity-based fluorescence probes: properties and applications.RSC medicinal chemistry · 2021Review
- Article
- A Cysteine-Reactive Small Photo-Crosslinker Possessing Caged-Fluorescence Properties: Binding-Site Determination of a Combinatorially-Selected Peptide by Fluorescence Imaging/Tandem Mass Spectrometry.International journal of molecular sciences · 2018Article
- Rapid detection ofScience translational medicine · 2018Article
- Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.Journal of molecular biology · 2016Article
- A structural remedy toward bright dipolar fluorophores in aqueous media.Chemical science · 2015Article
- Solvatoemissive dual fluorescence of N-(pyridylmethyl)-3-nitro-1,8-naphthalimides.Journal of fluorescence · 2014Article
- Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates.The journal of physical chemistry. B · 2013Article
- Attachable solvatochromic fluorophores and bioconjugation studies.Organic letters · 2013Article
- FRET-capture: a sensitive method for the detection of dynamic protein interactions.Chembiochem : a European journal of chemical biology · 2013Article
- Chemical tools for studying directed cell migration.ACS chemical biology · 2011Review
- Increased structural flexibility at the active site of a fluorophore-conjugated beta-lactamase distinctively impacts its binding toward diverse cephalosporin antibiotics.The Journal of biological chemistry · 2011Article
- Development of a fluorogenic sensor for activated Cdc42.Bioorganic & medicinal chemistry letters · 2011Article
- Monitoring protein interactions and dynamics with solvatochromic fluorophores.Trends in biotechnology · 2010Review
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2 authors.
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Abstract
The solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide (4-DMN) possesses extremely sensitive emission properties due largely to the low intrinsic fluorescence it exhibits in polar protic solvents such as water. This makes it well suited as a probe for the detection of a wide range of biomolecular interactions. Herein we report the development and evaluation of a new series of thiol-reactive agents derived from this fluorophore. The members of this series vary according to linker type and the electrophilic group required for the labeling of proteins and other biologically relevant molecules. Using the calcium-binding protein calmodulin as a model system, we compare the performance of the 4-DMN derivatives to that of several commercially available solvatochromic fluorophores identifying many key factors important to the successful application of such tools. This study also demonstrates the power of this new series of labeling agents by yielding a fluorescent calmodulin construct capable of producing a greater than 100-fold increase in emission intensity upon binding to calcium.
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