ReviewTransgenic research2010
Zinc-finger nucleases: a powerful tool for genetic engineering of animals.
Review in Transgenic research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 140 citations in OpenAlex.
- Synthetic Biology-Based Engineering Cells for Drug Delivery.Exploration (Beijing, China) · 2025Review
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- Review
- How genome editing changed the world of large animal research.Frontiers in genome editing · 2023Review
- Transgenesis and Genome Engineering: A Historical Review.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Historical DNA Manipulation Overview.Methods in molecular biology (Clifton, N.J.) · 2022Article
- How CRISPR-Cas System Could Be Used to Combat Antimicrobial Resistance.Infection and drug resistance · 2020Review
- Recent advances in mammalian reproductive biology.Science China. Life sciences · 2020Review
- Application of genome editing technologies in rats for human disease models.Journal of human genetics · 2018Review
- Generation of gene-edited rats by delivery of CRISPR/Cas9 protein and donor DNA into intact zygotes using electroporation.Scientific reports · 2017Article
- A history of genome editing in mammals.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish.Scientific reports · 2016Article
- Class II major histocompatibility complex mutant mice to study the germ-line bias of T-cell antigen receptors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A non-inheritable maternal Cas9-based multiple-gene editing system in mice.Scientific reports · 2016Article
- Zinc Finger Nuclease: A New Approach to Overcome Beta-Lactam Antibiotic Resistance.Jundishapur journal of microbiology · 2016Article
- Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.Scientific reports · 2015Article
- Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes.PloS one · 2015Article
- Mouse Genome Editing Using the CRISPR/Cas System.Current protocols in human genetics · 2014Article
- The new CRISPR-Cas system: RNA-guided genome engineering to efficiently produce any desired genetic alteration in animals.Transgenic research · 2014Review
- A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice.BMC biotechnology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The generation of genetically modified animals or plants with gene-targeted deletions or modifications is a powerful tool to analyze gene function, study disease and produce organisms of economical interest. Until recently, the generation of animals with gene targeted manipulations has been accomplished by homologous recombination (HR) in embryonic stem (ES) cells or cloning through nuclear transfer and has been limited to a few species. Recently, a new technology based on the use of gene-targeted zinc-finger nucleases (ZFNs) was developed and used for the generation of organisms with gene-targeted deletions and/or modifications when combined with HR. ZFNs have been used to generate modified organisms such as plants, Drosophila, zebra fish and rats with gene-targeted mutations. This perspective manuscript is a short review on the use of ZFNs for the genetic engineering of plants and animals, with particular emphasis on our recent work involving rats. We also discuss the application of other targeted nucleases, including homing endonucleases. Microinjection of plasmid or mRNA for ZFNs into rat embryos allowed targeted, rapid, complete, permanent and heritable disruption of endogenous loci. The application of ZFNs to generate gene-targeted knockouts in species where ES cells or cloning techniques are not available is an important new development to answer fundamental biological questions and develop models of economical interest such as for the production of humanized antibodies. Further refinements of ZFN technology in combination with HR may allow knock-ins in early embryos even in species where ES cells or cloning techniques are available.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.