ArticleNature methods2009
A general life-death selection strategy for dissecting protein functions.
Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Three decades of protein-fragment complementation.Nature reviews. Molecular cell biology · 2025Article
- Intra-Frontiers in genetics · 2023Article
- Controllable genome editing with split-engineered base editors.Nature chemical biology · 2021Article
- Protein-fragment complementation assays for large-scale analysis of protein-protein interactions.Biochemical Society transactions · 2021Review
- FRETting about the affinity of bimolecular protein-protein interactions.Protein science : a publication of the Protein Society · 2018Article
- Dynamic protein assembly by programmable DNA strand displacement.Nature chemistry · 2018Article
- Extended Linkers Improve the Detection of Protein-protein Interactions (PPIs) by Dihydrofolate Reductase Protein-fragment Complementation Assay (DHFR PCA) in Living Cells.Molecular & cellular proteomics : MCP · 2018Article
- Protein interaction perturbation profiling at amino-acid resolution.Nature methods · 2017Article
- EC-tagging allows cell type-specific RNA analysis.Nucleic acids research · 2017Article
- A method for high-throughput production of sequence-verified DNA libraries and strain collections.Molecular systems biology · 2017Article
- A Split-Ubiquitin Based Strategy Selecting for Protein Complex-Interfering Mutations.G3 (Bethesda, Md.) · 2016Article
- Review
- An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.Nature methods · 2014Article
- Exploration of the dynamic properties of protein complexes predicted from spatially constrained protein-protein interaction networks.PLoS computational biology · 2014Article
- Dissection of Cdk1-cyclin complexes in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- All or nothing: protein complexes flip essentiality between distantly related eukaryotes.Genome biology and evolution · 2013Article
- Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Annotator: postprocessing software for generating function-based signatures from quantitative mass spectrometry.Journal of proteome research · 2012Article
- Evidence for the robustness of protein complexes to inter-species hybridization.PLoS genetics · 2012Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Clonal selection strategies are central tools in molecular biology. We developed a general strategy to dissect protein functions through positive and negative clonal selection for protein-protein interactions, based on a protein-fragment complementation assay using Saccharomyces cerevisiae cytosine deaminase as a reporter. We applied this method to mutational or chemical disruption of protein-protein interactions in yeast and to dissection of the functions of an allosterically activated transcription factor, Swi6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.