ArticleNucleic acids research2009
Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Bacteriophage-related epigenetic natural and non-natural pyrimidine nucleotides and their influence on transcription with T7 RNA polymerase.Communications chemistry · 2024Article
- Epigenetic Pyrimidine Nucleotides in Competition with Natural dNTPs as Substrates for Diverse DNA Polymerases.ACS chemical biology · 2022Article
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- Influence of major-groove chemical modifications of DNA on transcription by bacterial RNA polymerases.Nucleic acids research · 2016Article
- Synthesis and Enzymatic Incorporation of Modified Deoxyuridine Triphosphates.Molecules (Basel, Switzerland) · 2015Article
- Effects of 2'-O-methyl nucleotide substitution on EcoRI endonuclease cleavage activities.PloS one · 2013Article
- Enzymatic cleavage of type II restriction endonucleases on the 2'-O-methyl nucleotide and phosphorothioate substituted DNA.PloS one · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of 6 base-modified 2'-deoxyadenosine derivatives was incorporated to diverse DNA sequences by primer extension using Vent (exo-) polymerase and the influence of the modification on cleavage by diverse restriction endonucleases was studied. While 8-substituted (Br or methyl) adenine derivatives were well tolerated by the restriction enzymes and the corresponding sequences were cleaved, the presence of 7-substituted 7-deazaadenine in the recognition sequence resulted in blocking of cleavage by some enzymes depending on the nature and size of the 7-substituent. All sequences with modifications outside of the recognition sequence were perfectly cleaved by all the restriction enzymes. The results are useful both for protection of some sequences from cleavage and for manipulation of functionalized DNA by restriction cleavage.
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Registered trials
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