ReviewJournal of biomechanics2010
Combining mechanical and optical approaches to dissect cellular mechanobiology.
Review in Journal of biomechanics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Field Guide to Traction Force Microscopy.Cellular and molecular bioengineering · 2024Review
- Sounding a New Era in Biomechanics with Acoustic Force Spectroscopy.Advances in experimental medicine and biology · 2023Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Force- and cell state-dependent recruitment of Piezo1 drives focal adhesion dynamics and calcium entry.Science advances · 2022Article
- Tutorial on laser speckle rheology: technology, applications, and opportunities.Journal of biomedical optics · 2020Review
- Engineered Biomaterial Platforms to Study Fibrosis.Advanced healthcare materials · 2020Review
- High-throughput microfluidic micropipette aspiration device to probe time-scale dependent nuclear mechanics in intact cells.Lab on a chip · 2019Article
- Mechanical Forces in Cutaneous Wound Healing: Emerging Therapies to Minimize Scar Formation.Advances in wound care · 2018Review
- Zinc oxide nanorod field effect transistor for long-time cellular force measurement.Scientific reports · 2017Article
- Review of cellular mechanotransduction on micropost substrates.Medical & biological engineering & computing · 2016Review
- The structure and function of the pericellular matrix of articular cartilage.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- Mechanotransduction and fibrosis.Journal of biomechanics · 2014Review
- Optical measurement of arterial mechanical properties: from atherosclerotic plaque initiation to rupture.Journal of biomedical optics · 2013Review
- Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage.Journal of the Royal Society, Interface · 2012Article
- On the role of DNA biomechanics in the regulation of gene expression.Journal of the Royal Society, Interface · 2011Review
- Article
- Contractility dominates adhesive ligand density in regulating cellular de-adhesion and retraction kinetics.Annals of biomedical engineering · 2011Article
- Mechanical properties of cellularly responsive hydrogels and their experimental determination.Advanced materials (Deerfield Beach, Fla.) · 2010Review
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Mechanical force modulates a wide array of cell physiological processes. Cells sense and respond to mechanical stimuli using a hierarchy of structural complexes spanning multiple length scales, including force-sensitive molecules and cytoskeletal networks. Understanding mechanotransduction, i.e., the process by which cells convert mechanical inputs into biochemical signals, has required the development of novel biophysical tools that allow for probing of cellular and subcellular components at requisite time, length, and force scales and technologies that track the spatio-temporal dynamics of relevant biomolecules. In this review, we begin by discussing the underlying principles and recent applications of atomic force microscopy, magnetic twisting cytometry, and traction force microscopy, three tools that have been widely used for measuring the mechanical properties of cells and for probing the molecular basis of cellular mechanotransduction. We then discuss how such tools can be combined with advanced fluorescence methods for imaging biochemical processes in living cells in the context of three specific problem spaces. We first focus on fluorescence resonance energy transfer, which has enabled imaging of intra- and inter-molecular interactions and enzymatic activity in real time based on conformational changes in sensor molecules. Next, we examine the use of fluorescence methods to probe force-dependent dynamics of focal adhesion proteins. Finally, we discuss the use of calcium ratiometric signaling to track fast mechanotransductive signaling dynamics. Together, these studies demonstrate how single-cell biomechanical tools can be effectively combined with molecular imaging technologies for elucidating mechanotransduction processes and identifying mechanosensitive proteins.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.