ArticleAnalytical biochemistry2010
Use of a semisynthetic epitope to probe histidine kinase activity and regulation.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Immunoblot-based activity assay for heme-containing histidine kinases.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2026Article
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- Insights into the structure and function of the histidine kinase ComP from Bacillus amyloliquefaciens based on molecular modeling.Bioscience reports · 2022Article
- The W-Acidic Motif of Histidine Kinase WalK Is Required for Signaling and Transcriptional Regulation inFrontiers in microbiology · 2022Article
- The many ways that nature has exploited the unusual structural and chemical properties of phosphohistidine for use in proteins.The Biochemical journal · 2021Review
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- Mechanistic Studies of Bioorthogonal ATP Analogues for Assessment of Histidine Kinase Autophosphorylation.ACS chemical biology · 2020Article
- Article
- Advances in development of new tools for the study of phosphohistidine.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- An O2-sensing stressosome from a Gram-negative bacterium.Nature communications · 2016Article
- Nitric Oxide Mediates Biofilm Formation and Symbiosis in Silicibacter sp. Strain TrichCH4B.mBio · 2015Article
- The Cutting Edge of Affinity Electrophoresis Technology.Proteomes · 2015Review
- A pan-specific antibody for direct detection of protein histidine phosphorylation.Nature chemical biology · 2013Article
- Intramolecular arrangement of sensor and regulator overcomes relaxed specificity in hybrid two-component systems.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Activity-based probe for histidine kinase signaling.Journal of the American Chemical Society · 2012Article
- Chasing phosphohistidine, an elusive sibling in the phosphoamino acid family.ACS chemical biology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Histidine-aspartic acid phosphotransfer pathways are central components of prokaryotic signal transduction pathways and are also found in many eukaryotes. Tools to study histidine kinases, however, are currently quite limited. In this article, we present a new tool to study histidine-aspartic acid phosphotransfer pathways. We show that many histidine kinases will accept ATPgammaS as a substrate to form a stable thiophosphohistidine even when they do not form stable phosphohistidines using the natural substrate ATP. An antibody that has previously been used to detect thiophosphorylated serine, threonine, and tyrosine residues is shown to recognize thiophosphohistidine and thiophosphoaspartic acid residues. Histidine kinase autothiophosphorylation is regulated by other protein sensor domains in the same way as autophosphorylation, and thiophosphate is transferred to downstream aspartic acid containing response regulators.
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Registered trials
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