ArticleJournal of immunological methods2009
Improved serum-free culture conditions for the differentiation of human and murine fibrocytes.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 71 citations in OpenAlex.
- Signal-integrated control of immune and reparative programs establishes blood-derived fibrocytes reprogrammable toward induced pluripotency.Biology open · 2026Article
- Role of Chitinase 3-like-1 in Myelofibrosis via Fibroblast-Produced Extracellular Matrix Enhancement.Journal of cellular physiology · 2026Article
- Pharmacological inhibition of host pathways enhances macrophage killing of intracellular bacterial pathogens.Microbiology spectrum · 2026Article
- Inhibition of Talin2 dedifferentiates myofibroblasts and reverses lung and kidney fibrosis.Scientific reports · 2025Article
- Mesangiogenic progenitor cells: a mesengenic and vasculogenic branch of hemopoiesis? A story of neglected plasticity.Frontiers in cell and developmental biology · 2025Article
- Blocking antibodies against integrin-α3, -αM, and -αMβ2 de-differentiate myofibroblasts, and improve lung fibrosis and kidney fibrosis.Scientific reports · 2024Article
- Gallein and isoniazid act synergistically to attenuatebioRxiv : the preprint server for biology · 2024Article
- Gallein potentiates isoniazid's ability to suppressFrontiers in microbiology · 2024Article
- Characterization of Circulating Fibrocytes in People Living with HIV on Stable Antiretroviral Therapy.ImmunoHorizons · 2022Article
- GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes.Cell death & disease · 2022Article
- Inhibiting Sialidase-Induced TGF-The Journal of pharmacology and experimental therapeutics · 2021Article
- Polyphosphate is an extracellular signal that can facilitate bacterial survival in eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Reduced Sialylation and Bioactivity of the Antifibrotic Protein Serum Amyloid P in the Sera of Patients with Idiopathic Pulmonary Fibrosis.ImmunoHorizons · 2020Article
- Attenuated pulmonary fibrosis in sialidase-3 knockout (American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Extracellular Polyphosphate Promotes Macrophage and Fibrocyte Differentiation, Inhibits Leukocyte Proliferation, and Acts as a Chemotactic Agent for Neutrophils.Journal of immunology (Baltimore, Md. : 1950) · 2019Article
- Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential.Science advances · 2019Article
- KJournal of the American Heart Association · 2019Article
- Effects of CTLA4-Ig treatment on circulating fibrocytes and skin fibroblasts from the same systemic sclerosis patients: an in vitro assay.Arthritis research & therapy · 2018Article
- Altered pattern of monocyte differentiation and monocyte-derived TGF-β1 in severe asthma.Scientific reports · 2018Article
- C-reactive protein (CRP) but not the related pentraxins serum amyloid P and PTX3 inhibits the proliferation and induces apoptosis of the leukemia cell line Mono Mac 6.BMC immunology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
CD14+ peripheral blood monocytes can differentiate into fibroblast-like cells called fibrocytes. Fibrocytes are associated with, and are at least partially responsible for, wound healing and fibrosis in multiple organ systems. In a variety of lesions in vivo, monocytes appear to differentiate into fibrocytes within days. However, in vitro culture conditions can take up to two weeks to generate fibrocytes. In this study, we describe enhanced serum-free conditions that support the rapid differentiation of human and murine fibrocytes. We compared the effect on fibrocyte differentiation of different anti-coagulants used when collecting blood, and for culturing cells, the effects of different commercial media formulations, the addition of a variety of supplements, cell density, conditioned medium, and glass and plastic substrates. We found that both heparin and EDTA were suitable anti-coagulants, but that blood treated with citrate-phosphate dextrose led to a reduced number of fibrocytes. Fibrocyte differentiation was enhanced when the serum-free medium was based on either FibroLife or StemPro formulations. We also found that only positively charged or hydrophilic glass and plastic surfaces provide adequate support for fibrocyte differentiation. Finally, the optimal cell density was 2.5 x 10(5)cells/ml (approximately 800 cells per mm(2)). These results indicate that blood collection, substrates, media, and cell density all influence in vitro fibrocyte differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.