ArticleJournal of controlled release : official journal of the Controlled Release Society2010
Intracellular delivery and calcium transients generated in sonoporation facilitated by microbubbles.
Article in Journal of controlled release : official journal of the Controlled Release Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Alteration of Essential Cell Function by Ultrasound-Targeted Microbubble Cavitation.Current cardiology reports · 2025Review
- CaUltrasonics sonochemistry · 2025Article
- Shear Stress and Microbubble-Mediated Modulation of Endothelial Cell Immunobiology.Small science · 2025Article
- Focused ultrasound in modern medicine: bioengineering interfaces, molecular effects, and clinical breakthroughs.Frontiers in bioengineering and biotechnology · 2025Review
- The molecular impact of sonoporation: A transcriptomic analysis of gene regulation profile.Ultrasonics sonochemistry · 2024Article
- Dynamic reorganization of multivesicular bodies and exosome production impacted by sonoporation.Scientific reports · 2024Article
- Shear stress preconditioning and microbubble flow pattern modulate ultrasound-assisted plasma membrane permeabilization.Materials today. Bio · 2024Article
- Development of an In Vitro Model to Study Mechanisms of Ultrasound-Targeted Microbubble Cavitation-Mediated Blood-Brain Barrier Opening.Ultrasound in medicine & biology · 2024Article
- A multicellular brain spheroid model for studying the mechanisms and bioeffects of ultrasound-enhanced drug penetration beyond the blood‒brain barrier.Scientific reports · 2024Article
- Article
- Landscape of Cellular Bioeffects Triggered by Ultrasound-Induced Sonoporation.International journal of molecular sciences · 2022Review
- The cellular response to plasma membrane disruption for nanomaterial delivery.Nano convergence · 2022Review
- Ultrasound-Mediated Drug Delivery: Sonoporation Mechanisms, Biophysics, and Critical Factors.BME frontiers · 2022Review
- Ultrasound-Triggered Liposomes Encapsulating Quantum Dots as Safe Fluorescent Markers for Colorectal Cancer.Pharmaceutics · 2021Article
- Precise Ultrasound Neuromodulation in a Deep Brain Region Using Nano Gas Vesicles as Actuators.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications.Beilstein journal of nanotechnology · 2021Review
- Synergistic ultrasonic biophysical effect-responsive nanoparticles for enhanced gene delivery to ovarian cancer stem cells.Drug delivery · 2020Article
- Focused Ultrasound for Immunomodulation of the Tumor Microenvironment.Journal of immunology (Baltimore, Md. : 1950) · 2020Review
- Visualization and quantification of dynamic intercellular coupling in human embryonic stem cells using single cell sonoporation.Scientific reports · 2020Article
- Transendothelial Perforations and the Sphere of Influence of Single-Site Sonoporation.Ultrasound in medicine & biology · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Ultrasound application in the presence of microbubbles is a promising strategy for intracellular drug and gene delivery, but it may also trigger other cellular responses. This study investigates the relationship between the change of cell membrane permeability generated by ultrasound-driven microbubbles and the changes in intracellular calcium concentration ([Ca(2+)](i)). Cultured rat cardiomyoblast (H9c2) cells were exposed to a single ultrasound pulse (1MHz, 10-15cycles, 0.27MPa) in the presence of a Definity(TM) microbubble. Intracellular transport via sonoporation was assessed in real time using propidium iodide (PI), while [Ca(2+)](i) and dye loss from the cells were measured with preloaded fura-2. The ultrasound exposure generated fragmentation or shrinking of the microbubble. Only cells adjacent to the ultrasound-driven microbubble exhibited propidium iodide uptake with simultaneous [Ca(2+)](i) increase and fura-2 dye loss. The amount of PI uptake was correlated with the amount of fura-2 dye loss. Cells with delayed [Ca(2+)](i) transients from the time of ultrasound application had no uptake of PI. These results indicate the formation of non-specific pores in the cell membrane by ultrasound-stimulated microbubbles and the generation of calcium waves in surrounding cells without pores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.