ArticleAnalytical biochemistry2010
A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Article in Analytical biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- p21-Positive Senescent Stromal Cells Promote Prostate Cancer Immune Suppression and Progression That Can Be Reversed by Senolytic Therapy.Cancer discovery · 2026Article
- Recent Advances in Biosensor Technology for Early-Stage Detection of Hepatocellular Carcinoma-Specific Biomarkers: An Overview.Diagnostics (Basel, Switzerland) · 2024Review
- Successful Preservation of Native BCR::ABL1 in Chronic Myeloid Leukemia Primary Leukocytes Reveals a Reduced Kinase Activity.Frontiers in oncology · 2022Article
- A Novel ELISA-Based Peptide Biosensor Assay for Screening ABL1 ActivityFrontiers in pharmacology · 2021Article
- Application of a Substrate-Mediated Selection with c-Src Tyrosine Kinase to a DNA-Encoded Chemical Library.Molecules (Basel, Switzerland) · 2019Article
- Assays for tyrosine phosphorylation in human cells.Methods in enzymology · 2019Article
- Monitoring Chronic Myeloid Leukemia: How Molecular Tools May Drive Therapeutic Approaches.Frontiers in oncology · 2019Review
- A Cell-Based Assay for Measuring Endogenous BcrAbl Kinase Activity and Inhibitor Resistance.PloS one · 2016Article
- KINATEST-ID: a pipeline to develop phosphorylation-dependent terbium sensitizing kinase assays.Journal of the American Chemical Society · 2015Article
- Global analysis of human nonreceptor tyrosine kinase specificity using high-density peptide microarrays.Journal of proteome research · 2014Article
- Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells.Angewandte Chemie (International ed. in English) · 2013Article
- Time-resolved luminescence detection of spleen tyrosine kinase activity through terbium sensitization.Analytical chemistry · 2013Article
- A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor.PloS one · 2013Article
- A peptide photoaffinity probe specific for the active conformation of the Abl tyrosine kinase.Chembiochem : a European journal of chemical biology · 2012Article
- A peptide-based biosensor assay to detect intracellular Syk kinase activation and inhibition.Biochemistry · 2012Article
- Detection of early Abl kinase activation after ionizing radiation by using a peptide biosensor.Chembiochem : a European journal of chemical biology · 2012Article
- Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine.The Journal of biological chemistry · 2012Article
- A general strategy for studying multisite protein phosphorylation using label-free selected reaction monitoring mass spectrometry.Analytical biochemistry · 2011Article
- Photocleavable peptide-conjugated magnetic beads for protein kinase assays by MALDI-TOF MS.Bioconjugate chemistry · 2010Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Many cancers are characterized by changes in protein phosphorylation as a result of kinase dysregulation. Disruption of Abl kinase signaling through the Philadelphia chromosome (causing the Bcr-Abl mutation) in chronic myeloid leukemia (CML) has provided a paradigm for development of kinase inhibitor drugs such as the specific inhibitor imatinib (also known as STI571 or Gleevec). However, because patients are treated indefinitely with this drug to maintain remission, resistance is increasingly becoming an issue. Although there are many ways to detect kinase activity, most lack the ability to "multiplex" the analysis (i.e., to detect more than one substrate simultaneously). Here we report a novel biosensor for detecting Abl kinase activity and sensitivity to inhibitor in live intact cells overexpressing a CML model Abl kinase construct. This straightforward methodology could eventually provide a new tool for detecting kinase activity and inhibitor drug response in cancer cells that overexpress oncogenic kinases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.