ArticleFEMS microbiology letters2009
Legionella pneumophila secretes an endoglucanase that belongs to the family-5 of glycosyl hydrolases and is dependent upon type II secretion.
Article in FEMS microbiology letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
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- Structure, Dynamics and Cellular Insight Into Novel Substrates of theFrontiers in molecular biosciences · 2020Article
- Type II Secretion Promotes Bacterial Growth within theInfection and immunity · 2019Article
- Assessing the impact, genomics and evolution of type II secretion across a large, medically important genus: the Legionella type II secretion paradigm.Microbial genomics · 2019Article
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- Type II Secretion Substrates ofmBio · 2017Article
- Expanding Role of Type II Secretion in Bacterial Pathogenesis and Beyond.Infection and immunity · 2017Review
- Life Stage-specific Proteomes of Legionella pneumophila Reveal a Highly Differential Abundance of Virulence-associated Dot/Icm effectors.Molecular & cellular proteomics : MCP · 2016Article
- Current and past strategies for bacterial culture in clinical microbiology.Clinical microbiology reviews · 2015Review
- The novel Legionella pneumophila type II secretion substrate NttC contributes to infection of amoebae Hartmannella vermiformis and Willaertia magna.Microbiology (Reading, England) · 2014Article
- Metabolism of the vacuolar pathogen Legionella and implications for virulence.Frontiers in cellular and infection microbiology · 2014Article
- Multiple Legionella pneumophila Type II secretion substrates, including a novel protein, contribute to differential infection of the amoebae Acanthamoeba castellanii, Hartmannella vermiformis, and Naegleria lovaniensis.Infection and immunity · 2013Article
- Legionella cardiaca sp. nov., isolated from a case of native valve endocarditis in a human heart.International journal of systematic and evolutionary microbiology · 2012Article
- The major facilitator superfamily-type protein LbtC promotes the utilization of the legiobactin siderophore by Legionella pneumophila.Microbiology (Reading, England) · 2012Article
- Genome analyses highlight the different biological roles of cellulases.Nature reviews. Microbiology · 2012Review
- Functional characterization of EpsC, a component of the type II secretion system, in the pathogenicity of Vibrio vulnificus.Infection and immunity · 2011Article
- Lcl of Legionella pneumophila is an immunogenic GAG binding adhesin that promotes interactions with lung epithelial cells and plays a crucial role in biofilm formation.Infection and immunity · 2011Article
- Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epithelia.Infection and immunity · 2011Article
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2 authors at 1 institution in 1 country.
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Abstract
Examination of cell-free culture supernatants revealed that Legionella pneumophila strains secrete an endoglucanase activity. Legionella pneumophila lspF mutants were deficient for this activity, indicating that the endoglucanase is secreted by the bacterium's type II protein secretion (T2S) system. Inactivation of celA, encoding a member of the family-5 of glycosyl hydrolases, abolished the endoglucanase activity in L. pneumophila culture supernatants. The cloned celA gene conferred activity upon recombinant Escherichia coli. Thus, CelA is the major secreted endoglucanase of L. pneumophila. Mutants inactivated for celA grew normally in protozoa and macrophage, indicating that CelA is not required for the intracellular phase of L. pneumophila. The CelA endoglucanase is one of at least 25 proteins secreted by the type II system of L. pneumophila and the 17th type of enzyme effector associated with this pathway. Only a subset of the other Legionella species tested expressed secreted endoglucanase activity, suggesting that the T2S output differs among the different legionellae. Overall, this study represents the first documentation of an endoglucanase (EC 3.2.1.4) being produced by a strain of Legionella.
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