ArticleBiomacromolecules2009
Smooth muscle cell phenotype modulation and contraction on native and cross-linked polyelectrolyte multilayers.
Article in Biomacromolecules, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- Modulation of Smooth Muscle Cell Phenotype for Translation of Tissue-Engineered Vascular Grafts.Tissue engineering. Part B, Reviews · 2023Review
- Photoelasticity-based evaluation of cellular contractile force for phenotypic discrimination of vascular smooth muscle cells.Scientific reports · 2019Article
- Primary Culture of Rat Aortic Vascular Smooth Muscle Cells: A New Method.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Collective epithelial cell sheet adhesion and migration on polyelectrolyte multilayers with uniform and gradients of compliance.Experimental cell research · 2016Article
- Deletion of Periostin Protects Against Atherosclerosis in Mice by Altering Inflammation and Extracellular Matrix Remodeling.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- A comparative study of two different approaches for the incorporation of silver nanoparticles into layer-by-layer films.Nanoscale research letters · 2014Article
- MEMS-based shear characterization of soft hydrated samples.Journal of micromechanics and microengineering : structures, devices, and systems · 2013Article
- Rigidity-patterned polyelectrolyte films to control myoblast cell adhesion and spatial organization.Advanced functional materials · 2013Article
- Cell durotaxis on polyelectrolyte multilayers with photogenerated gradients of modulus.Biomacromolecules · 2013Article
- Perivascular cells in blood vessel regeneration.Biotechnology journal · 2013Review
- Homogeneity, modulus, and viscoelasticity of polyelectrolyte multilayers by nanoindentation: refining the buildup mechanism.Langmuir : the ACS journal of surfaces and colloids · 2012Article
- Cytotoxicity of free versus multilayered polyelectrolytes.Biomacromolecules · 2011Article
- Electrochemically addressed cross-links in polyelectrolyte multilayers: cyclic duravoltammetry.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Indentation versus tensile measurements of Young's modulus for soft biological tissues.Tissue engineering. Part B, Reviews · 2011Review
- Correlating the compliance and permeability of photo-cross-linked polyelectrolyte multilayers.Langmuir : the ACS journal of surfaces and colloids · 2011Article
- Polyelectrolyte multilayers in tissue engineering.Tissue engineering. Part B, Reviews · 2011Review
- Human coronary artery smooth muscle cell responses to bioactive polyelectrolyte multilayer interfaces.Biotechnology research international · 2011Article
- Molecular regulation of contractile smooth muscle cell phenotype: implications for vascular tissue engineering.Tissue engineering. Part B, Reviews · 2010Review
- Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Smooth muscle cells convert between a motile, proliferative "synthetic" phenotype and a sessile, "contractile" phenotype. The ability to manipulate the phenotype of aortic smooth muscle cells with thin biocompatible polyelectrolyte multilayers (PEMUs) with common surface chemical characteristics but varying stiffness was investigated. The stiffness of (PAH/PAA) PEMUs was varied by heating to form covalent amide bond cross-links between the layers. Atomic force microscopy (AFM) showed that cross-linked PEMUs were thinner than those that were not cross-linked. AFM nanoindentation demonstrated that the Young's modulus ranged from 6 MPa for hydrated native PEMUs to more than 8 GPa for maximally cross-linked PEMUs. Rat aortic A7r5 smooth muscle cells cultured on native PEMUs exhibited morphology and motility of synthetic cells and expression of the synthetic phenotype markers vimentin, tropomyosin 4, and nonmuscle myosin heavy chain IIB (nmMHCIIB). In comparison, cells cultured on maximally cross-linked PEMUs exhibited the phenotype markers calponin, smooth muscle myosin heavy chain (smMHC), myocardin, transgelin, and smooth muscle alpha-actin (smActin) that are characteristic of the smooth muscle "contractile" phenotype. Consistent with those cells being "contractile", A7r5 cells grown on cross-linked PEMUs produced contractile force when stimulated with a Ca(2+) ionophore.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.