ArticleMolecular biology reports2010
Molecular and biochemical analysis of the beta class carbonic anhydrases in Caenorhabditis elegans.
Article in Molecular biology reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Article
- Anion inhibition studies of a beta carbonic anhydrase from the malaria mosquito Anopheles gambiae.Journal of enzyme inhibition and medicinal chemistry · 2018Article
- Involvement of β-Carbonic Anhydrase Genes in Bacterial Genomic Islands and Their Horizontal Transfer to Protists.Applied and environmental microbiology · 2018Article
- Innovative molecular diagnosis of Trichinella species based on β-carbonic anhydrase genomic sequence.Microbial biotechnology · 2016Article
- Carbonic Anhydrases in Cnidarians: Novel Perspectives from the Octocorallian Corallium rubrum.PloS one · 2016Article
- A Novel Mechanism of pH Buffering in C. elegans Glia: Bicarbonate Transport via the Voltage-Gated ClC Cl- Channel CLH-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Ascaris lumbricoides β carbonic anhydrase: a potential target enzyme for treatment of ascariasis.Parasites & vectors · 2015Article
- The Evolutionary History of Daphniid α-Carbonic Anhydrase within Animalia.International journal of evolutionary biology · 2015Article
- Bioinformatic analysis of beta carbonic anhydrase sequences from protozoans and metazoans.Parasites & vectors · 2014Article
- Article
- Molecular and biochemical analysis of the α class carbonic anhydrases in Caenorhabditis elegans.Molecular biology reports · 2011Article
- Sulfurous gases as biological messengers and toxins: comparative genetics of their metabolism in model organisms.Journal of toxicology · 2011Article
- Characterization of the first beta-class carbonic anhydrase from an arthropod (Drosophila melanogaster) and phylogenetic analysis of beta-class carbonic anhydrases in invertebrates.BMC biochemistry · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The beta class of the carbonic anhydrase (CA) enzyme family has been found in plants, yeast, bacteria and algae, but not in animals. Also, little is known concerning the CAs of C. elegans. Genes possibly encoding beta-CAs were revealed by in silico analysis of the C. elegans genome. Amino acid sequence and 3D structure analysis revealed a resemblance to both plant and cab-type beta-CAs. Temporal expression patterns of the two genes, as well as changes in expression levels under different atmospheric conditions (stress) were analyzed by real-time RT-PCR. Recombinant enzymes, expressed in E. coli were used for in vitro measurement of CA activity, while a yeast complementation experiment was performed in order to assess their ability to complement the function of S. crevisieae beta-CA (NCE103) in vivo. RNAi by feeding was performed on wild-type populations that were then examined for a visible phenotype under normal or various stress conditions (pH, CO(2)/O(2)). Two genes possibly encoding beta-CAs were revealed (bca-1 and y116a8c.28). Their products contain elements of both plant and cab-type CAs. Both assays showed that Y116a8c.28 is an active CA. Both genes showed significant levels of transcript accumulation during development, while they also responded to the stress conditions. No visible phenotype was scored under normal or stress conditions.
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