ArticleCellular and molecular neurobiology2010
Pilocarpine protects cobalt chloride-induced apoptosis of RGC-5 cells: involvement of muscarinic receptors and HIF-1 alpha pathway.
Article in Cellular and molecular neurobiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Muscarinic Acetylcholine Receptors in the Retina-Therapeutic Implications.International journal of molecular sciences · 2021Review
- Paeonol Induces Protective Autophagy in Retinal Photoreceptor Cells.Frontiers in pharmacology · 2021Article
- Glaucoma as Neurodegeneration in the Brain.Eye and brain · 2021Review
- Anti-inflammatory and antioxidant effects of muscarinic acetylcholine receptor (mAChR) activation in the rat hippocampus.Scientific reports · 2019Article
- Overview of Bile Acids Signaling and Perspective on the Signal of Ursodeoxycholic Acid, the Most Hydrophilic Bile Acid, in the Heart.Biomolecules · 2018Review
- Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells.Molecular medicine reports · 2018Article
- [G protein-coupled receptor 17 is involved in CoClZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2018Article
- Pigment epithelium-derived factor protects retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction.International journal of ophthalmology · 2017Article
- Effect of the M1 Muscarinic Acetylcholine Receptor on Retinal Neuron Number Studied with Gene-Targeted Mice.Journal of molecular neuroscience : MN · 2015Article
- Oxidative stress induces autophagy in response to multiple noxious stimuli in retinal ganglion cells.Autophagy · 2014Review
- Activation of muscarinic receptors protects against retinal neurons damage and optic nerve degeneration in vitro and in vivo models.CNS neuroscience & therapeutics · 2014Article
- Dulbecco's Modified Eagle Medium is neuroprotective when compared to standard vitrectomy irrigation solution.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2013Article
- Stanniocalcin-1 protects retinal ganglion cells by inhibiting apoptosis and oxidative damage.PloS one · 2013Article
- N-acetylcysteine protects against hypoxia mimetic-induced autophagy by targeting the HIF-1α pathway in retinal ganglion cells.Cellular and molecular neurobiology · 2012Article
- Hypoxia induces apoptosis and autophagic cell death in human periodontal ligament cells through HIF-1α pathway.Cell proliferation · 2012Article
- YC-1 targeting of hypoxia-inducible factor-1α reduces RGC-5 cell viability and inhibits cell proliferation.Molecular vision · 2012Article
- Mechanisms and biology of B-cell leukemia/lymphoma 2/adenovirus E1B interacting protein 3 and Nip-like protein X.Antioxidants & redox signaling · 2011Review
- Neuroprotective effect of peroxiredoxin 6 against hypoxia-induced retinal ganglion cell damage.BMC neuroscience · 2010Article
- Lutein protects RGC-5 cells against hypoxia and oxidative stress.International journal of molecular sciences · 2010Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The retina is the most metabolically active tissue in the human body and hypoxia-induced retinal ganglion cell (RGC) death has been implicated in glaucomatous optic neuropathy. The aim of this study is to determine whether muscarinic receptor agonist pilocarpine, a classic antiglaucoma drug, possesses neuroprotection against cobalt chloride (CoCl(2))-mimetic hypoxia-induced apoptosis of rat retinal ganglion cells (RGC-5 cells) and its underlying mechanisms. Cell viability was determined by Cell Counting Kit-8 assay and apoptosis was examined by annexin V and mitochondrial membrane potential (MMP) assays. Expressions of hypoxia-induced factor-1 alpha (HIF-1 alpha), p53, and BNIP3 were investigated by quantitative real-time PCR and western blot analysis. After treatment of 200 microM CoCl(2) for 24 h, RGC-5 cells showed a marked decrease of cell viability by approximately 30%, increased apoptosis rate and obvious decline in MMP, which could largely be reversed by the pretreatment of 1 microM pilocarpine mainly via the activation of muscarinic receptors. Meanwhile, pretreatment of 1 microM pilocarpine could significantly prevent CoCl(2)-induced HIF-1 alpha translocation from cytoplasm to nucleus and down-regulate the expression of HIF-1 alpha, p53, and BNIP3. These studies demonstrated that pilocarpine had effective protection against hypoxia-induced apoptosis in RGCs via muscarinic receptors and HIF-1 alpha pathway. The findings suggest that HIF-1 alpha pathway as a "master switch" may be used as a therapeutic target in the cholinergic treatment of glaucoma.
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