ArticleParticle and fibre toxicology2009
Diesel exhaust particles modulate the tight junction protein occludin in lung cells in vitro.
Article in Particle and fibre toxicology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Impact of airborne particulate matter on skin: a systematic review from epidemiology to in vitro studies.Particle and fibre toxicology · 2020Pooled it
- Article
- An Online Measurement Approach to Monitor the Deposition of Diesel Exhaust Particles on Lung Cells In Vitro.Environmental science & technology · 2025Article
- Air Pollution and Skin Diseases.Annals of dermatology · 2025Review
- The New Paradigm: The Role of Proteins and Triggers in the Evolution of Allergic Asthma.International journal of molecular sciences · 2024Review
- Exploring the immunopathology of type 2 inflammatory airway diseases.Frontiers in immunology · 2024Review
- Carbon nanoparticles adversely affect CFTR expression and toxicologically relevant pathways.Scientific reports · 2022Article
- Short-term exposure of PMFrontiers in public health · 2022Article
- Interactions of nasal epithelium with macrophages and dendritic cells variously alter urban PM-induced inflammation in healthy, asthma and COPD.Scientific reports · 2021Article
- Effects of ozone and particulate matter on airway epithelial barrier structure and function: a review ofInhalation toxicology · 2021Review
- Measurement of Airway Epithelial Permeability: Methods and Protocols.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Effects of Particulate Matter in a Mouse Model of Oxazolone-Induced Atopic Dermatitis.Annals of dermatology · 2020Article
- Dendritic Cells: Critical Regulators of Allergic Asthma.International journal of molecular sciences · 2020Review
- Diesel exhaust particle exposure reduces expression of the epithelial tight junction protein Tricellulin.Particle and fibre toxicology · 2020Article
- Exposure Effects Beyond the Epithelial Barrier: Transepithelial Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung Fibroblasts in an Organotypic Human Airway Model.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Titanium dioxide nanoparticles exaggerate respiratory syncytial virus-induced airway epithelial barrier dysfunction.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Metal particles in mucus and hypertrophic tissue of the inferior nasal turbinates from the human upper respiratory tract.Environmental science and pollution research international · 2020Article
- Epithelial barriers in allergy and asthma.The Journal of allergy and clinical immunology · 2020Review
- Mechanism of ambient particulate matter and respiratory infections.Journal of thoracic disease · 2020Article
- Potential role of polycyclic aromatic hydrocarbons as mediators of cardiovascular effects from combustion particles.Environmental health : a global access science source · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUsing an in vitro triple cell co-culture model consisting of human epithelial cells (16HBE14o-), monocyte-derived macrophages and dendritic cells, it was recently demonstrated that macrophages and dendritic cells create a transepithelial network between the epithelial cells to capture antigens without disrupting the epithelial tightness. The expression of the different tight junction proteins in macrophages and dendritic cells, and the formation of tight junction-like structures with epithelial cells has been demonstrated. Immunofluorescent methods combined with laser scanning microscopy and quantitative real-time polymerase chain reaction were used to investigate if exposure to diesel exhaust particles (DEP) (0.5, 5, 50, 125 mug/ml), for 24 h, can modulate the expression of the tight junction mRNA/protein of occludin, in all three cell types.
resultsOnly the highest dose of DEP (125 mug/ml) seemed to reduce the occludin mRNA in the cells of the defence system however not in epithelial cells, although the occludin arrangement in the latter cell type was disrupted. The transepithelial electrical resistance was reduced in epithelial cell mono-cultures but not in the triple cell co-cultures, following exposure to high DEP concentration. Cytotoxicity was not found, in either epithelial mono-cultures nor in triple cell co-cultures, after exposure to the different DEP concentrations.
conclusionWe concluded that high concentrations of DEP (125 mug/ml) can modulate the tight junction occludin mRNA in the cells of the defence system and that those cells play an important role maintaining the epithelial integrity following exposure to particulate antigens in lung cells.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.