ArticleBritish journal of pharmacology2009
Vitamin D inhibits growth of human airway smooth muscle cells through growth factor-induced phosphorylation of retinoblastoma protein and checkpoint kinase 1.
Article in British journal of pharmacology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 164 citations in OpenAlex.
- The Role of Vitamin D Supplementation on Airway Remodeling in Asthma: A Systematic Review.Nutrients · 2023Pooled it
- Trial
- VITALITY - a longitudinal cohort established to assess the role of postnatal vitamin D supplementation in atopic disease and immune health.European journal of epidemiology · 2026Article
- The Role of Vitamin D3 in Ocular Diseases.Nutrients · 2024Review
- Vitamin D and allergic diseases.Frontiers in immunology · 2024Review
- Assessment of the Relation Between Asthma Severity and Serum Vitamin D Levels: A Cross-Sectional Study.Cureus · 2023Article
- Effect of Obesity on Lung Function in the Pediatric and Adult Populations with Asthma: A Review.Journal of clinical medicine · 2023Review
- The Role of Vitamins in the Pathogenesis of Asthma.International journal of molecular sciences · 2023Review
- Maternal vitamin D status modifies the effects of early life tobacco exposure on child lung function.The Journal of allergy and clinical immunology · 2023Article
- Risk Factors Affecting Development and Persistence of Preschool Wheezing: Consensus Document of the Emilia-Romagna Asthma (ERA) Study Group.Journal of clinical medicine · 2022Article
- Metabolomics in asthma: A platform for discovery.Molecular aspects of medicine · 2022Review
- Observational
- Vitamin D Deficiency in Development: How Much Is Enough, and How Much Is Too Much?American journal of respiratory cell and molecular biology · 2021Article
- Low gestational vitamin D level and childhood asthma are related to impaired lung function in high-risk children.The Journal of allergy and clinical immunology · 2021Article
- Milk Consumption and Respiratory Function in Asthma Patients: NHANES Analysis 2007-2012.Nutrients · 2021Article
- Review
- Vitamin D3 Attenuates Viral-Induced Inflammation and Fibrotic Responses in Bronchial Smooth Muscle Cells.Frontiers in immunology · 2021Article
- Prenatal vitamin D supplementation and child respiratory health: A systematic review and meta-analysis of randomized controlled trials.The World Allergy Organization journal · 2020Review
- Article
- Status and prospects: personalized treatment and biomarker for airway remodeling in asthma.Journal of thoracic disease · 2020Review
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
background and purposeAirway remodelling in asthma is manifested, in part, as increased airway smooth muscle (ASM) mass, reflecting myocyte proliferation. We hypothesized that calcitriol, a secosteroidal vitamin D receptor (VDR) modulator, would inhibit growth factor-induced myocyte proliferation. EXPERIMENTAL APPROACH: Human ASM cell cultures were derived from bronchial samples taken during surgery. ASM cells were treated with platelet-derived growth factor (PDGF) (10 ng.mL(-1)) for 24 h in the presence of calcitriol, dexamethasone or a checkpoint kinase 1 (Chk1) inhibitor (SB218078). The effects of calcitriol on PDGF-mediated cell proliferation were assessed by thymidine incorporation assay, propidium iodide-based cell cycle analysis, caspase-3 assay and immunoblotting for specific cell cycle modulators. KEY
resultsCalcitriol, but not dexamethasone, inhibited PDGF-induced ASM DNA synthesis concentration dependently (IC(50)= 520 +/- 52 nM). These effects were associated with VDR-mediated expression of cytochrome CYP24A1 with no effects on ASM apoptosis. Calcitriol substantially inhibited (P < 0.01) PDGF-stimulated cell growth in ASM derived from both normal (59 +/- 8%) and asthmatic subjects (57 +/- 9%). Calcitriol inhibited PDGF-induced phosphorylation of retinoblastoma protein (Rb) and Chk1, with no effects on PDGF-mediated activation of extracellular signal-regulated kinases 1/2, PI3-kinase and S6 kinase, or expression of p21(Waf/Cip-1), p27(Kip1), cyclin D and E2F-1. Consistent with these observations, SB218078 also inhibited (IC(50)= 450 +/- 100 pM) PDGF-induced cell cycle progression. CONCLUSIONS AND IMPLICATIONS: Calcitriol decreased PDGF-induced ASM cell growth by inhibiting Rb and Chk1 phosphorylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.