ArticleGenesis (New York, N.Y. : 2000)2009
Dual fluorescent protein reporters for studying cell behaviors in vivo.
Article in Genesis (New York, N.Y. : 2000), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed.
- Human stem cell-specific epigenetic signatures control transgene expression.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024Article
- Toxicity of nuclear-localized GFP in reporter mice.Scientific reports · 2024Article
- Review
- Article
- Expression of a membrane-targeted fluorescent reporter disrupts auditory hair cell mechanoelectrical transduction and causes profound deafness.Hearing research · 2021Article
- CreLite: An optogenetically controlled Cre/loxP system using red light.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissue.Skeletal muscle · 2020Article
- Non-Cell-Autonomous Mechanisms in Radial Projection Neuron Migration in the Developing Cerebral Cortex.Frontiers in cell and developmental biology · 2020Review
- Myocardial differentiation is dependent upon endocardial signaling during early cardiogenesisDevelopment (Cambridge, England) · 2019Article
- Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins.Genesis (New York, N.Y. : 2000) · 2019Article
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
- Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multisample Format.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency.Developmental cell · 2017Article
- Generation of a transgenic mouse line for conditional expression of human IL-6.Experimental animals · 2016Article
- Computer simulations of the mouse spermatogenic cycle.Biology open · 2014Article
- Par3 controls neural crest migration by promoting microtubule catastrophe during contact inhibition of locomotion.Development (Cambridge, England) · 2013Article
- A Uchl1-Histone2BmCherry:GFP-gpi BAC transgene for imaging neuronal progenitors.Genesis (New York, N.Y. : 2000) · 2013Article
- Wnt3 function in the epiblast is required for the maintenance but not the initiation of gastrulation in mice.Developmental biology · 2013Article
- A pink mouse reports the switch from red to green fluorescence upon Cre-mediated recombination.BMC research notes · 2012Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Fluorescent proteins (FPs) are useful tools for visualizing live cells and their behaviors. Protein domains that mediate subcellular localization have been fused to FPs to highlight cellular structures. FPs fused with histone H2B incorporate into chromatin allowing visualization of nuclear events. FPs fused to a glycosylphosphatidylinositol anchor signal sequence label the plasma membrane, highlighting cellular shape. Thus, a reporter gene containing both types of FP fusions would allow for effective monitoring of cell shape, movement, mitotic stage, apoptosis, and other cellular activities. Here, we report a binary color-coding system using four differently colored FP reporters that generates 16 distinct color codes to label the nuclei and plasma membranes of live cells in culture and in transgenic mice. As an initial test of this system in vivo, the promoter of the human Ubiquitin C (UBC) gene was used to widely express one of the color-code reporters. Widespread expression of the reporter was attained in embryos; however, both male and female transgenic mice were infertile. In contrast, the promoter of the mouse Oct4/Pou5f1 gene linked to two different color-code reporters specifically labeled blastocysts, primordial germ cells, and postnatal germ cells, and these mice were fertile. Time-lapse movies of fluorescently-labeled primordial germs cells demonstrate the utility of the color-code system to visualize cell behaviors. This set of new FP reporters should be a useful tool for labeling distinct cell populations and studying their behaviors in complex tissues in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.